YAL002W S000000002 VPS8 Membrane-associated hydrophilic protein that interacts with the small GTPase, Vps21p, to facilitate soluble vacuolar protein localization; required for localization and trafficking of the CPY sorting receptor; contains a RING finger motif ORF Verified viable YAL004W S000002136 Hypothetical protein ORF Dubious viable YAL005C S000000004 SSA1 ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport; member of heat shock protein 70 (HSP70) family; forms a chaperone complex with Ydj1p; localized to the nucleus, cytoplasm, and cell wall ORF Verified viable YAL007C S000000005 ERP2 Protein that forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles ORF Verified viable YAL008W S000000006 FUN14 Mitochondrial protein of unknown function ORF Verified viable YAL009W S000000007 SPO7 Integral nuclear/ER membrane protein of unknown function, required for normal nuclear envelope morphology and sporulation ORF Verified viable YAL010C S000000008 MDM10 Subunit of the mitochondrial sorting and assembly machinery (SAM complex); has a role in assembly of the TOM complex, which mediates protein import through the outer membrane; required for normal mitochondrial morphology and inheritance ORF Verified viable YAL011W S000000009 SWC3 Protein of unknown function, component of the Swr1p complex that incorporates Htz1p into chromatin; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae ORF Verified viable YAL012W S000000010 CYS3 Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; ORF Verified viable YAL013W S000000011 DEP1 Transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation ORF Verified viable YAL014C S000000012 SYN8 Endosomal SNARE related to mammalian syntaxin 8 ORF Verified viable YAL015C S000000013 NTG1 DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase involved in base excision repair, localizes to the nucleus and mitochondrion ORF Verified viable YAL016W S000000014 TPD3 Regulatory subunit A of the heterotrimeric protein phosphatase 2A, which also contains regulatory subunit Cdc55p and either catalytic subunit Pph21p or Pph22p; required for cell morphogenesis and for transcription by RNA polymerase III ORF Verified viable YAL017W S000000015 PSK1 One of two (see also PSK2) PAS domain containing S/T protein kinases; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status ORF Verified viable YAL018C S000000016 Hypothetical protein ORF Uncharacterized viable YAL019W S000000017 FUN30 Protein whose overexpression affects chromosome stability, potential Cdc28p substrate; homolog of Snf2p ORF Verified viable YAL020C S000000018 ATS1 Protein with a potential role in regulatory interactions between microtubules and the cell cycle, as suggested by genetic and physical interactions with Nap1p and genetic interactions with TUB1 ORF Verified viable YAL021C S000000019 CCR4 Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening ORF Verified viable YAL022C S000000020 FUN26 Nucleoside transporter with broad nucleoside selectivity; localized to intracellular membranes ORF Verified viable YAL023C S000000021 PMT2 Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt1p, can instead interact with Pmt5p in some conditions; target for new antifungals ORF Verified viable YAL026C S000000024 DRS2 Integral membrane Ca(2+)-ATPase involved in aminophospholipid translocation; required to form a specific class of secretory vesicles that accumulate upon actin cytoskeleton disruption; mutation affects maturation of the 18S rRNA ORF Verified viable YAL027W S000000025 Hypothetical protein ORF Uncharacterized viable YAL028W S000000026 FRT2 Tail-anchored endoplasmic reticulum membrane protein, interacts with homolog Frt1p but is not a substrate of calcineurin (unlike Frt1p), promotes growth in conditions of high Na+, alkaline pH, or cell wall stress; potential Cdc28p substrate ORF Verified viable YAL029C S000000027 MYO4 One of two type V myosins; required for mother-specific HO expression, for the bud tip localization of ASH1 and IST2 mRNA; facilitates growth and orientation of ER tubules along with She3p ORF Verified viable YAL030W S000000028 SNC1 Vesicle membrane receptor protein (v-SNARE) involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins ORF Verified viable YAL031C S000000029 GIP4 Cytoplasmic Glc7-interacting protein whose overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; potential Cdc28p substrate ORF Verified viable YAL034C S000002134 FUN19 Non-essential protein of unknown function ORF Verified viable YAL035W S000000033 FUN12 GTPase, required for general translation initiation by promoting Met-tRNAiMet binding to ribosomes and ribosomal subunit joining; homolog of bacterial IF2 ORF Verified viable YAL036C S000000034 RBG1 Member of the DRG family of GTP-binding proteins; interacts with translating ribosomes and with Tma46p ORF Verified viable YAL037W S000000035 Hypothetical protein ORF Uncharacterized viable YAL039C S000000037 CYC3 Cytochrome c heme lyase (holocytochrome c synthase), attaches heme to apo-Cyc1p in the mitochondrial intermembrane space; human ortholog may have a role in microphthalmia with linear skin defects (MLS) ORF Verified viable YAL040C S000000038 CLN3 G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis ORF Verified viable YAL042C-A S000002138 Hypothetical protein ORF Dubious viable YAL042W S000000040 ERV46 Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport ORF Verified viable YAL044C S000000042 GCV3 H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm ORF Verified viable YAL045C S000000043 Hypothetical protein ORF Dubious viable YAL046C S000000044 Hypothetical protein ORF Uncharacterized viable YAL047C S000000045 SPC72 Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization ORF Verified viable YAL048C S000000046 GEM1 Evolutionarily-conserved tail-anchored outer mitochondrial membrane GTPase which regulates mitochondrial morphology; cells lacking Gem1p contain collapsed, globular, or grape-like mitochondria; not required for pheromone-induced cell death ORF Verified viable YAL049C S000000047 Hypothetical protein ORF Uncharacterized viable YAL051W S000000048 OAF1 Oleate-activated transcription factor, acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis ORF Verified viable YAL053W S000000049 FLC2 Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance ORF Verified viable YAL054C S000000050 ACS1 Acetyl-coA synthetase isoform, expressed during growth on nonfermentable carbon sources and under aerobic conditions ORF Verified viable YAL055W S000000051 PEX22 Putative peroxisomal membrane protein required for import of peroxisomal proteins, functionally complements a Pichia pastoris pex22 mutation ORF Verified viable YAL056C-A S000002139 Hypothetical protein ORF Dubious viable YAL056W S000000052 GPB2 Proposed beta subunit of the heterotrimeric G protein; interacts with the receptor Grp1p, affects signaling by altering the level of phosphorylation of PKS substrates; regulates pseudohyphal growth through cAMP levels; homolog of Gpb1p ORF Verified viable YAL058W S000000054 CNE1 Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast ORF Verified viable YAL059W S000000055 ECM1 Protein of unknown function, localized in the nucleoplasm and the nucleolus, genetically interacts with MTR2 in 60S ribosomal protein subunit export ORF Verified viable YAL060W S000000056 BDH1 NAD-dependent (2R,3R)-2,3-butanediol dehydrogenase, a zinc-containing medium-chain alcohol dehydrogenase, produces 2,3-butanediol from acetoin during fermentation and allows using 2,3-butanediol as a carbon source during aerobic growth ORF Verified viable YAL061W S000000057 putative polyol dehydrogenase ORF Uncharacterized viable YAL062W S000000058 GDH3 NADP(+)-dependent glutamate dehydrogenase, synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources ORF Verified viable YAL064C-A S000002140 Hypothetical protein ORF Uncharacterized viable YAL065C S000001817 Hypothetical protein ORF Uncharacterized viable YAL066W S000000061 Hypothetical protein ORF Dubious viable YAL067C S000000062 SEO1 Putative permease, member of the allantoate transporter subfamily of the major facilitator superfamily; mutation confers resistance to ethionine sulfoxide ORF Verified viable YAL068C S000002142 PAU8 Hypothetical protein ORF Uncharacterized viable YAR002C-A S000002129 ERP1 Protein that forms a heterotrimeric complex with Erp2p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles ORF Verified viable YAR002W S000000063 NUP60 Subunit of the nuclear pore complex (NPC), functions to anchor Nup2p to the NPC in a process controlled by the nucleoplasmic concentration of Gsp1p-GTP; potential Cdc28p substrate; involved in telomere maintenance ORF Verified viable YAR003W S000000064 SWD1 Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7 ORF Verified viable YAR014C S000000069 BUD14 Protein involved in bud-site selection, Bud14p-Glc7p complex functions as a cortical regulator of dynein; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern ORF Verified viable YAR015W S000000070 ADE1 N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine ORF Verified viable YAR018C S000000071 KIN3 Nonessential protein kinase with unknown cellular role ORF Verified viable YAR020C S000000073 PAU7 Part of 23-member seripauperin multigene family, active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme ORF Verified viable YAR023C S000000074 Putative integral membrane protein, member of DUP240 gene family ORF Uncharacterized viable YAR027W S000000075 UIP3 Putative integral membrane protein of unknown function; interacts with Ulp1p at the nuclear periphery; member of DUP240 gene family ORF Verified viable YAR028W S000000076 Putative integral membrane protein, member of DUP240 gene family ORF Uncharacterized viable YAR029W S000000077 Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern ORF Uncharacterized viable YAR030C S000001821 Hypothetical protein ORF Dubious viable YAR031W S000000078 PRM9 Pheromone-regulated protein with 3 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; member of DUP240 gene family ORF Verified viable YAR035W S000000080 YAT1 Outer mitochondrial carnitine acetyltransferase, minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix ORF Verified viable YAR042W S000000081 SWH1 Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction ORF Verified viable YAR047C S000000083 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data ORF Dubious viable YAR050W S000000084 FLO1 Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p ORF Verified viable YBL001C S000000097 ECM15 Non-essential protein of unknown function, likely exists as tetramer, may be regulated by the binding of small-molecule ligands (possibly sulfate ions), may have a role in yeast cell-wall biogenesis ORF Verified viable YBL002W S000000098 HTB2 One of two nearly identical (see HTB1) histone H2B subtypes required for chromatin assembly and chromosome function; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation ORF Verified viable YBL003C S000000099 HTA2 One of two nearly identical (see also HTA1) histone H2A subtypes; core histone required for chromatin assembly and chromosome function; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p ORF Verified viable YBL005W S000000101 PDR3 Transcriptional activator of the pleiotropic drug resistance network, regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting sites known as PDREs (PDR responsive elements) ORF Verified viable YBL006C S000000102 LDB7 Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions ORF Verified viable YBL007C S000000103 SLA1 Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; contains 3 SH3 domains; interacts with proteins regulating actin dynamics and with proteins required for endocytosis ORF Verified viable YBL008W S000000104 HIR1 Non-essential transcriptional corepressor involved in the cell cycle-regulated transcription of histone H2A, H2B, H3 and H4 genes; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores ORF Verified viable YBL009W S000000105 haspin ORF Uncharacterized viable YBL010C S000000106 Hypothetical protein ORF Uncharacterized viable YBL011W S000000107 SCT1 Glycerol 3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase of the glycerolipid biosynthesis pathway, prefers 16-carbon fatty acids, similar to Gpt2p, gene is constitutively transcribed ORF Verified viable YBL012C S000000108 Hypothetical protein ORF Dubious viable YBL013W S000000109 FMT1 Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate ORF Verified viable YBL015W S000000111 ACH1 Acetyl-coA hydrolase, primarily localized to mitochondria; required for acetate utilization and for diploid pseudohyphal growth ORF Verified viable YBL016W S000000112 FUS3 Mitogen-activated protein kinase involved in mating pheromone response; activated by phoshporylation by Ste7p; provides specificity during the mating vs. filamentous growth response by phosphorylating transcriptional and cytoplasmic targets ORF Verified viable YBL017C S000000113 PEP1 Type I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golgi and prevacuolar endosome-like compartments ORF Verified viable YBL019W S000000115 APN2 Class II abasic (AP) endonuclease involved in repair of DNA damage; homolog of human HAP1 and E. coli exoIII ORF Verified viable YBL021C S000000117 HAP3 Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding ORF Verified viable YBL022C S000000118 PIM1 Mitochondrial ATP-dependent protease involved in intramitochondrial proteolysis; involved in degradation of misfolded proteins in mitochondria; required for bigenesis and maintenance of mitochondria ORF Verified viable YBL024W S000000120 NCL1 S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; similar to Nop2p and human proliferation associated nucleolar protein p120 ORF Verified viable YBL025W S000000121 RRN10 Protein involved in promoting high level transcription of rDNA, subunit of UAF (upstream activation factor) for RNA polymerase I ORF Verified viable YBL027W S000000123 RPL19B Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl19Ap and has similarity to rat L19 ribosomal protein; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal ORF Verified viable YBL028C S000000124 Hypothetical protein ORF Uncharacterized viable YBL029W S000000125 Hypothetical protein ORF Uncharacterized viable YBL031W S000000127 SHE1 Cytoskeletal protein of unknown function; overexpression causes growth arrest ORF Verified viable YBL032W S000000128 HEK2 RNA binding protein with similarity to hnRNP-K that localizes to the cytoplasm and to subtelomeric DNA; required for the proper localization of ASH1 mRNA; involved in the regulation of telomere position effect and telomere length ORF Verified viable YBL033C S000000129 RIB1 GTP cyclohydrolase II; catalyzes the first step of the riboflavin biosynthesis pathway ORF Verified viable YBL036C S000000132 Single-domain racemase, possibly non-specific due to the lack of the second domain, which presumably determines specificity ORF Verified viable YBL037W S000000133 APL3 Alpha-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport ORF Verified viable YBL038W S000000134 MRPL16 Mitochondrial ribosomal protein of the large subunit ORF Verified viable YBL039C S000000135 URA7 Major CTP synthase isozyme (see also URA8), catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis ORF Verified viable YBL042C S000000138 FUI1 High affinity uridine permease, localized to the plasma membrane; not involved in uracil transport ORF Verified viable YBL043W S000000139 ECM13 Non-essential protein of unknown function ORF Verified viable YBL044W S000000140 Hypothetical protein ORF Uncharacterized viable YBL045C S000000141 COR1 Core subunit of the ubiquinol-cytochrome c reductase complex (bc1 complex), which is a component of the mitochondrial inner membrane electron transport chain ORF Verified viable YBL046W S000000142 PSY4 Putative regulatory subunit of an evolutionarily conserved protein phosphatase complex containing the catalytic subunit Pph3p and a third subunit Psy2p; required for cisplatin resistance; GFP-fusion protein localizes to the nucleus ORF Verified viable YBL047C S000000143 EDE1 Key endocytic protein involved in a network of interactions with other endocytic proteins, binds membranes in a ubiquitin-dependent manner, may also bind ubiquitinated membrane-associated proteins ORF Verified viable YBL048W S000000144 Hypothetical protein ORF Dubious viable YBL049W S000000145 MOH1 Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for viability in stationary phase ORF Uncharacterized viable YBL051C S000000147 PIN4 Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage ORF Verified viable YBL052C S000000148 SAS3 Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; sas3 gcn5 double mutation confers lethality ORF Verified viable YBL053W S000000149 Hypothetical protein ORF Dubious viable YBL054W S000000150 Hypothetical protein ORF Uncharacterized viable YBL055C S000000151 Hypothetical protein ORF Uncharacterized viable YBL056W S000000152 PTC3 Type 2C protein phosphatase; dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA checkpoint inactivation ORF Verified viable YBL057C S000000153 PTH2 One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for cell growth ORF Verified viable YBL058W S000000154 SHP1 UBX (ubiquitin regulatory X) domain-containing protein that regulates Glc7p phosphatase activity and interacts with Cdc48p; interacts with ubiquitylated proteins in vivo and is required for degradation of a ubiquitylated model substrate ORF Verified viable YBL059W S000000155 Hypothetical protein ORF Uncharacterized viable YBL060W S000000156 Hypothetical protein ORF Uncharacterized viable YBL061C S000000157 SKT5 Activator of Chs3p (chitin synthase III), recruits Chs3p to the bud neck via interaction with Bni4p; has similarity to Shc1p, which activates Chs3p during sporulation ORF Verified viable YBL062W S000000158 Hypothetical protein ORF Dubious viable YBL063W S000000159 KIP1 Kinesin-related motor protein required for mitotic spindle assembly and chromosome segregation; functionally redundant with Cin8p ORF Verified viable YBL064C S000000160 PRX1 Mitochondrial peroxiredoxin (1-Cys Prx) with thioredoxin peroxidase activity, has a role in reduction of hydroperoxides; induced during respiratory growth and under conditions of oxidative stress ORF Verified viable YBL065W S000000161 Hypothetical protein ORF Dubious viable YBL066C S000000162 SEF1 Putative transcription factor, has homolog in Kluyveromyces lactis ORF Verified viable YBL067C S000000163 UBP13 Putative ubiquitin-specific protease ORF Verified viable YBL068W S000000164 PRS4 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, involved in nucleotide, histidine, and tryptophan biosynthesis; one of a five related enzymes, which are active as heteromultimeric complexes ORF Verified viable YBL069W S000000165 AST1 Peripheral membrane protein that interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane, possibly by influencing its incorporation into lipid rafts ORF Verified viable YBL070C S000000166 Hypothetical protein ORF Dubious viable YBL071C S000000167 Hypothetical protein ORF Dubious viable YBL072C S000000168 RPS8A Protein component of the small (40S) ribosomal subunit; identical to Rps8Bp and has similarity to rat S8 ribosomal protein ORF Verified viable YBL075C S000000171 SSA3 ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm ORF Verified viable YBL078C S000000174 ATG8 Protein required for autophagy; modified by the serial action of Atg4p, Atg7p, and Atg3p, and conjugated at the C terminus with phosphatidylethanolamine, to become the form essential for generation of autophagosomes ORF Verified viable YBL079W S000000175 NUP170 Abundant subunit of the nuclear pore complex (NPC), required for proper localization of specific nucleoporins within the NPC, involved in nuclear envelope permeability and in chromosome segregation, has similarity to Nup157p ORF Verified viable YBL080C S000000176 PET112 Protein required for mitochondrial translation; mutation is functionally complemented by a Bacillus subtilis ortholog ORF Verified viable YBL081W S000000177 Hypothetical protein ORF Uncharacterized viable YBL082C S000000178 ALG3 Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins ORF Verified viable YBL083C S000000179 Hypothetical protein ORF Dubious viable YBL085W S000000181 BOI1 Protein implicated in polar growth, functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain ORF Verified viable YBL086C S000000182 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery ORF Uncharacterized viable YBL087C S000000183 RPL23A Protein component of the large (60S) ribosomal subunit, identical to Rpl23Bp and has similarity to E. coli L14 and rat L23 ribosomal proteins ORF Verified viable YBL088C S000000184 TEL1 Protein kinase, primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; homolog of human ataxia telangiectasia (ATM) gene ORF Verified viable YBL089W S000000185 AVT5 Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters ORF Verified viable YBL090W S000000186 MRP21 Mitochondrial ribosomal protein of the large subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences ORF Verified viable YBL091C S000000187 MAP2 Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map1p ORF Verified viable YBL091C-A S000007228 SCS22 Protein involved in regulation of phospholipid metabolism; homolog of Scs2p2; similar to D. melanogaster inturned protein ORF Verified viable YBL093C S000000189 ROX3 RNA polymerase II holoenzyme component ORF Verified viable YBL094C S000000190 Hypothetical protein ORF Dubious viable YBL095W S000000191 Hypothetical protein ORF Uncharacterized viable YBL096C S000000192 Hypothetical protein ORF Dubious viable YBL098W S000000194 BNA4 Kynurenine 3-mono oxygenase, required for biosynthesis of nicotinic acid from tryptophan via kynurenine pathway ORF Verified viable YBL099W S000000195 ATP1 Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis ORF Verified viable YBL100C S000000196 Dubious open reading frame ORF Dubious viable YBL101C S000000197 ECM21 Non-essential protein of unknown function; promoter contains several Gcn4p binding elements ORF Verified viable YBL102W S000000198 SFT2 Non-essential tetra-spanning membrane protein found mostly in the late Golgi, can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5 ORF Verified viable YBL103C S000000199 RTG3 Basic helix-loop-helix-leucine zipper (bHLH/Zip) transcription factor that forms a complex with another bHLH/Zip protein, Rtg1p, to activate the retrograde (RTG) and TOR pathways ORF Verified viable YBL104C S000000200 Hypothetical protein ORF Uncharacterized viable YBL106C S000000202 SRO77 Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Sro7p and Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p ORF Verified viable YBL107C S000000203 Hypothetical protein ORF Uncharacterized viable YBR001C S000000205 NTH2 Putative neutral trehalase, required for thermotolerance and may mediate resistance to other cellular stresses ORF Verified viable YBR003W S000000207 COQ1 Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis ORF Verified viable YBR005W S000000209 RCR1 Protein of the endoplasmic reticulum membrane involved in chitin deposition in the cell wall; overproduction confers resistance to Congo Red ORF Verified viable YBR007C S000000211 DSF2 Deletion suppressor of mpt5 mutation ORF Uncharacterized viable YBR008C S000000212 FLR1 Plasma membrane multidrug transporter, member of the major facilitator superfamily; involved in efflux of fluconazole, diazaborine, benomyl, methotrexate, and other drugs ORF Verified viable YBR009C S000000213 HHF1 One of two identical histone H4 proteins (see also HHF2); core histone required for chromatin assembly and chromosome function; contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity ORF Verified viable YBR010W S000000214 HHT1 One of two identical histone H3 proteins (see also HHT2); core histone required for chromatin assembly, involved in heterochromatin-mediated telomeric and HM silencing; regulated by acetylation, methylation, and mitotic phosphorylation ORF Verified viable YBR012C S000000216 Hypothetical protein ORF Dubious viable YBR013C S000000217 Hypothetical protein ORF Uncharacterized viable YBR014C S000000218 Hypothetical protein ORF Uncharacterized viable YBR015C S000000219 MNN2 Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment ORF Verified viable YBR016W S000000220 Plasma membrane protein of unknown function; has similarity to hydrophilins, which are hydrophilic, glycine-rich proteins involved in the adaptive response to hyperosmotic conditions ORF Uncharacterized viable YBR017C S000000221 KAP104 Transportin, cytosolic karyopherin beta 2 involved in delivery of heterogeneous nuclear ribonucleoproteins to the nucleoplasm, binds rg-nuclear localization signals on Nab2p and Hrp1p, plays a role in cell-cycle progression ORF Verified viable YBR018C S000000222 GAL7 Galactose-1-phosphate uridyl transferase, synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism ORF Verified viable YBR019C S000000223 GAL10 UDP-glucose-4-epimerase, catalyzes the interconversion of UDP-galactose and UDP-D-glucose in galactose metabolism; also catalyzes the conversion of alpha-D-glucose or alpha-D-galactose to their beta-anomers ORF Verified viable YBR020W S000000224 GAL1 Galactokinase, phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p ORF Verified viable YBR021W S000000225 FUR4 Uracil permease, localized to the plasma membrane; expression is tightly regulated by uracil levels and environmental cues ORF Verified viable YBR022W S000000226 POA1 Phosphatase that is highly specific for ADP-ribose 1''-phosphate, a tRNA splicing metabolite; may have a role in regulation of tRNA splicing ORF Verified viable YBR023C S000000227 CHS3 Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan ORF Verified viable YBR024W S000000228 SCO2 Protein anchored to the mitochondrial inner membrane, similar to Sco1p and may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p ORF Verified viable YBR025C S000000229 Hypothetical protein ORF Uncharacterized viable YBR026C S000000230 ETR1 2-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis ORF Verified viable YBR027C S000000231 Hypothetical protein ORF Dubious viable YBR028C S000000232 Hypothetical protein ORF Uncharacterized viable YBR030W S000000234 Hypothetical protein ORF Uncharacterized viable YBR031W S000000235 RPL4A N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl4Bp and has similarity to E. coli L4 and rat L4 ribosomal proteins ORF Verified viable YBR032W S000000236 Hypothetical protein ORF Dubious viable YBR033W S000000237 EDS1 Non-essential protein of unknown function ORF Uncharacterized viable YBR034C S000000238 HMT1 Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, thus facilitating nuclear export of these proteins; required for viability of npl3 mutants ORF Verified viable YBR035C S000000239 PDX3 Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism ORF Verified viable YBR036C S000000240 CSG2 Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations ORF Verified viable YBR037C S000000241 SCO1 Copper-binding protein of the mitochondrial inner membrane, required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; has similarity to thioredoxins ORF Verified viable YBR039W S000000243 ATP3 Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis ORF Verified viable YBR040W S000000244 FIG1 Integral membrane protein required for efficient mating; may participate in or regulate the low affinity Ca2+ influx system, which affects intracellular signaling and cell-cell fusion during mating ORF Verified viable YBR041W S000000245 FAT1 Fatty acid transporter and very long-chain fatty acyl-CoA synthetase, may form a complex with Faa1p or Faa4p that imports and activates exogenous fatty acids ORF Verified viable YBR042C S000000246 Hypothetical protein ORF Uncharacterized viable YBR043C S000000247 QDR3 Multidrug transporter required for resistance to quinidine, barban, cisplatin, and bleomycin; member of the major facilitator superfamily of transporters conferring multiple drug resistance (MFS-MDR) ORF Verified viable YBR044C S000000248 TCM62 Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; putative chaperone ORF Verified viable YBR045C S000000249 GIP1 Meiosis-specific regulatory subunit of the Glc7p protein phosphatase, regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p ORF Verified viable YBR046C S000000250 ZTA1 Zeta-crystallin homolog, found in the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin, which has quinone oxidoreductase activity ORF Verified viable YBR047W S000000251 FMP23 The authentic, non-tagged protein was localized to the mitochondria ORF Uncharacterized viable YBR048W S000000252 RPS11B Protein component of the small (40S) ribosomal subunit; identical to Rps11Ap and has similarity to E. coli S17 and rat S11 ribosomal proteins ORF Verified viable YBR050C S000000254 REG2 Regulatory subunit of the Glc7p type-1 protein phosphatase; involved with Reg1p, Glc7p, and Snf1p in regulation of glucose-repressible genes, also involved in glucose-induced proteolysis of maltose permease ORF Verified viable YBR051W S000000255 Hypothetical protein ORF Dubious viable YBR052C S000000256 RFS1 Protein of unknown function; member of a flavodoxin-like fold protein family that includes Pst2p and Ycp4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern ORF Uncharacterized viable YBR053C S000000257 Hypothetical protein ORF Uncharacterized viable YBR054W S000000258 YRO2 Putative plasma membrane protein of unknown function, transcriptionally regulated by Haa1p; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and bud ORF Verified viable YBR056W S000000260 Hypothetical protein ORF Uncharacterized viable YBR057C S000000261 MUM2 Cytoplasmic protein essential for meiotic DNA replication and sporulation; interacts with Orc2p, which is a component of the origin recognition complex ORF Verified viable YBR058C S000000262 UBP14 Ubiquitin-specific protease that specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T ORF Verified viable YBR059C S000000263 AKL1 Ser-Thr protein kinase, member (with Ark1p and Prk1p) of the Ark kinase family; involved in endocytosis and actin cytoskeleton organization ORF Verified viable YBR061C S000000265 TRM7 2'-O-ribose methyltransferase, methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop ORF Verified viable YBR062C S000000266 Hypothetical protein ORF Uncharacterized viable YBR063C S000000267 Putative protein of unknown function; YBR063C is not an essential gene ORF Uncharacterized viable YBR064W S000000268 Hypothetical protein ORF Dubious viable YBR065C S000000269 ECM2 Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p ORF Verified viable YBR066C S000000270 NRG2 Transcriptional repressor that mediates glucose repression and negatively regulates filamentous growth; has similarity to Nrg1p ORF Verified viable YBR067C S000000271 TIP1 Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins ORF Verified viable YBR068C S000000272 BAP2 High-affinity leucine permease, functions as a branched-chain amino acid permease involved in the uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains ORF Verified viable YBR069C S000000273 TAT1 Amino acid transport protein for valine, leucine, isoleucine, and tyrosine, low-affinity tryptophan and histidine transporter; overexpression confers FK506 resistance ORF Verified viable YBR071W S000000275 Hypothetical protein ORF Uncharacterized viable YBR072W S000000276 HSP26 Small heat shock protein with chaperone activity that is regulated by a heat induced transition from an inactive oligomeric (24-mer) complex to an active dimer; induced by heat, upon entry into stationary phase, and during sporulation ORF Verified viable YBR073W S000000277 RDH54 DNA-dependent ATPase, stimulates strand exchange by modifying the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during mitosis and meiosis; proposed to be involved in crossover interference ORF Verified viable YBR074W S000000278 Putative metalloprotease ORF Uncharacterized viable YBR076W S000000280 ECM8 Non-essential protein of unknown function ORF Verified viable YBR077C S000000281 SLM4 Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4 ORF Verified viable YBR078W S000000282 ECM33 GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; similar to Sps2p and Pst1p ORF Verified viable YBR081C S000000285 SPT7 Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex ORF Verified viable YBR082C S000000286 UBC4 Ubiquitin-conjugating enzyme that mediates degradation of short-lived and abnormal proteins; interacts with E3-CaM in ubiquitinating calmodulin; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response ORF Verified viable YBR083W S000000287 TEC1 Transcription factor required for full Ty1 epxression, Ty1-mediated gene activation, and haploid invasive and diploid pseudohyphal growth; TEA/ATTS DNA-binding domain family member ORF Verified viable YBR084C-A S000002156 RPL19A Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl19Bp and has similarity to rat L19 ribosomal protein; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal ORF Verified viable YBR084W S000000288 MIS1 Mitochondrial C1-tetrahydrofolate synthase, involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase ORF Verified viable YBR085W S000000289 AAC3 Mitochondrial inner membrane ADP/ATP translocator, exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Pet9p and Aac1p; has roles in maintenance of viability and in respiration ORF Verified viable YBR086C S000000290 IST2 Plasma membrane protein that may be involved in osmotolerance, localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process ORF Verified viable YBR090C S000000294 Hypothetical protein ORF Uncharacterized viable YBR092C S000000296 PHO3 Constitutively expressed acid phosphatase similar to Pho5p; brought to the cell surface by transport vesicles; hydrolyzes thiamin phosphates in the periplasmic space, increasing cellular thiamin uptake; expression is repressed by thiamin ORF Verified viable YBR093C S000000297 PHO5 Repressible acid phosphatase (1 of 3) that also mediates extracellular nucleotide-derived phosphate hydrolysis; secretory pathway derived cell surface glycoprotein; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2 ORF Verified viable YBR094W S000000298 Hypothetical protein ORF Uncharacterized viable YBR095C S000000299 RXT2 Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth ORF Verified viable YBR096W S000000300 Hypothetical protein ORF Uncharacterized viable YBR097W S000000301 VPS15 Myristoylated serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p ORF Verified viable YBR098W S000000302 MMS4 Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination and DNA repair ORF Verified viable YBR099C S000000303 Hypothetical protein ORF Dubious viable YBR101C S000000305 FES1 Hsp70 (Ssa1p) nucleotide exchange factor, cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum ORF Verified viable YBR103W S000000307 SIF2 WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus ORF Verified viable YBR104W S000000308 YMC2 Putative mitochondrial inner membrane transporter, member of the mitochondrial carrier (MCF) family ORF Verified viable YBR105C S000000309 VID24 Peripheral membrane protein located at Vid (vacuole import and degradation) vesicles; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; involved in proteasome-dependent catabolite degradation of FBPase ORF Verified viable YBR106W S000000310 PHO88 Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations ORF Verified viable YBR107C S000000311 IML3 Protein with a role in kinetochore function, localizes to the outer kinetochore in a Ctf19p-dependent manner, interacts with Chl4p and Ctf19p ORF Verified viable YBR108W S000000312 Protein interacting with Rsv167p ORF Uncharacterized viable YBR111C S000000315 YSA1 Nudix hydrolase family member with ADP-ribose pyrophosphatase activity ORF Verified viable YBR112C S000000316 CYC8 General transcriptional co-repressor, acts together with Tup1p; also acts as part of a transcriptional co-activator complex that recruits the SWI/SNF and SAGA complexes to promoters ORF Verified viable YBR113W S000000317 Hypothetical protein ORF Dubious viable YBR114W S000000318 RAD16 Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4); member of the SWI/SNF family ORF Verified viable YBR115C S000000319 LYS2 Alpha aminoadipate reductase, catalyzes the reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is the fifth step in biosynthesis of lysine; activation requires posttranslational phosphopantetheinylation by Lys5p ORF Verified viable YBR116C S000000320 Hypothetical protein ORF Dubious viable YBR117C S000000321 TKL2 Transketolase, similar to Tkl1p; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids ORF Verified viable YBR118W S000000322 TEF2 Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes ORF Verified viable YBR119W S000000323 MUD1 U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing ORF Verified viable YBR120C S000000324 CBP6 Protein required for translation of the mitochondrial COB mRNA ORF Verified viable YBR122C S000000326 MRPL36 Mitochondrial ribosomal protein of the large subunit; overproduction suppresses mutations in the COX2 leader peptide-encoding region ORF Verified viable YBR125C S000000329 PTC4 Cytoplasmic type 2C protein phosphatase; identified as a high-copy number suppressor of the synthetic lethality of a cnb1 mpk1 double deletion; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity ORF Verified viable YBR126C S000000330 TPS1 Synthase subunit of trehalose-6-phosphate synthase/phosphatase complex, which synthesizes the storage carbohydrate trehalose; also found in a monomeric form; expression is induced by the stress response and repressed by the Ras-cAMP pathway ORF Verified viable YBR127C S000000331 VMA2 Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; contains nucleotide binding sites; also detected in the cytoplasm ORF Verified viable YBR128C S000000332 ATG14 Subunit of an autophagy-specific phosphatidylinositol 3-kinase complex (with Vps34p, Vps15p, and Vps30p) required for organization of a pre-autophagosomal structure; ATG14 transcription is activated by Gln3p during nitrogen starvation ORF Verified viable YBR129C S000000333 OPY1 Protein of unknown function, overproduction blocks cell cycle arrest in the presence of mating pheromone ORF Verified viable YBR130C S000000334 SHE3 Protein that acts as an adaptor between Myo4p and the She2p-mRNA complex; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; also required for cortical ER inheritance ORF Verified viable YBR131W S000000335 CCZ1 Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway ORF Verified viable YBR132C S000000336 AGP2 High affinity polyamine permease, preferentially uses spermidine over putrescine; expression is down-regulated by osmotic stress; plasma membrane carnitine transporter, also functions as a low-affinity amino acid permease ORF Verified viable YBR133C S000000337 HSL7 Protein arginine N-methyltransferase that exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p ORF Verified viable YBR134W S000000338 Hypothetical protein ORF Dubious viable YBR137W S000000341 Protein of unknown function; localized to the cytoplasm; binds to Replication Protein A (RPA); YBR137W is not an essential gene ORF Uncharacterized viable YBR138C S000000342 Cytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene ORF Uncharacterized viable YBR139W S000000343 Putative serine type Carboxypeptidase; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YBR139W is not an essential gene ORF Uncharacterized viable YBR141C S000000345 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene ORF Uncharacterized viable YBR144C S000000348 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR144C is not an essential gene ORF Dubious viable YBR145W S000000349 ADH5 Alcohol dehydrogenase isoenzyme V; involved in ethanol production ORF Verified viable YBR146W S000000350 MRPS9 Mitochondrial ribosomal protein of the small subunit ORF Verified viable YBR147W S000000351 Putative protein of unknown function; YBR147W is not an essential gene; resistant to fluconazole ORF Uncharacterized viable YBR148W S000000352 YSW1 Protein expressed specifically in spores ORF Verified viable YBR149W S000000353 ARA1 Large subunit of NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; small subunit is unidentified ORF Verified viable YBR150C S000000354 TBS1 Probable Zn-finger protein ORF Verified viable YBR151W S000000355 APD1 Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus ORF Verified viable YBR156C S000000360 SLI15 Subunit of the Ipl1p-Sli15p-Bir1p complex that regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly; regulates the activity and localization of the Ipl1p aurora kinase ORF Verified viable YBR157C S000000361 ICS2 Protein of unknown function; null mutation does not confer any obvious defects in growth, spore germination, viability, or carbohydrate utilization ORF Verified viable YBR158W S000000362 AMN1 Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN) ORF Verified viable YBR159W S000000363 IFA38 Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides. ORF Verified viable YBR161W S000000365 CSH1 Probable catalytic subunit of a mannosylinositol phosphorylceramide (MIPC) synthase, forms a complex with probable regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p ORF Verified viable YBR162C S000000366 TOS1 Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C ORF Uncharacterized viable YBR162W-A S000002158 YSY6 Protein whose expression suppresses a secretory pathway mutation in E. coli; has similarity to the mammalian RAMP4 protein involved in secretion ORF Verified viable YBR163W S000000367 DEM1 Protein of unknown function, shows similarity to RNA-processing protein Pta1p ORF Verified viable YBR164C S000000368 ARL1 Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor ORF Verified viable YBR165W S000000369 UBS1 Ubiquitin-conjugating enzyme suppressor that functions as a general positive regulator of Cdc34p activity; nuclear protein that may represent a link between nucleocytoplasmic transport and ubiquitin ligase activity ORF Verified viable YBR166C S000000370 TYR1 Prephenate dehydrogenase involved in tyrosine biosynthesis, expression is dependent on phenylalanine levels ORF Verified viable YBR168W S000000372 PEX32 Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p ORF Verified viable YBR169C S000000373 SSE2 Member of the heat shock protein 70 (HSP70) family; may be involved in protein folding; localized to the cytoplasm; highly homologous to the heat shock protein Sse1p ORF Verified viable YBR170C S000000374 NPL4 Endoplasmic reticulum and nuclear membrane protein, forms a complex with Cdc48p and Ufd1p that recognizes ubiquitinated proteins in the endoplasmic reticulum and delivers them to the proteasome for degradation ORF Verified viable YBR171W S000000375 SEC66 Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER ORF Verified viable YBR172C S000000376 SMY2 Protein of unknown function that interacts with Myo2p; has similarity to S. pombe Mpd2 ORF Verified viable YBR173C S000000377 UMP1 Short-lived chaperone required for correct maturation of the 20S proteasome; degraded by proteasome upon completion of its assembly; involved in ubiquitin-mediated proteolysis; mutant defective in degradation of short-lived proteins ORF Verified viable YBR174C S000000378 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective ORF Dubious viable YBR175W S000000379 SWD3 Essential subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5 ORF Verified viable YBR176W S000000380 ECM31 Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate ORF Verified viable YBR177C S000000381 EHT1 Acyl-coenzymeA:ethanol O-acyltransferase that plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; contains esterase activity; localizes to lipid particles and the mitochondrial outer membrane ORF Verified viable YBR178W S000000382 Hypothetical protein ORF Dubious viable YBR179C S000000383 FZO1 Mitochondrial integral membrane protein involved in mitochondrial fusion and maintenance of the mitochondrial genome; contains N-terminal GTPase domain ORF Verified viable YBR180W S000000384 DTR1 Multidrug resistance dityrosine transporter of the major facilitator superfamily, essential for spore wall synthesis, facilitates the translocation of bisformyl dityrosine through the prospore membrane ORF Verified viable YBR181C S000000385 RPS6B Protein component of the small (40S) ribosomal subunit; identical to Rps6Ap and has similarity to rat S6 ribosomal protein ORF Verified viable YBR182C S000000386 SMP1 Putative transcription factor involved in regulating the response to osmotic stress; member of the MADS-box family of transcription factors ORF Verified viable YBR183W S000000387 YPC1 Alkaline ceramidase that also has reverse (CoA-independent) ceramide synthase activity, catalyzes both breakdown and synthesis of phytoceramide; overexpression confers fumonisin B1 resistance ORF Verified viable YBR184W S000000388 Putative protein of unknown function; YBR184W is not an essential gene ORF Uncharacterized viable YBR185C S000000389 MBA1 Protein involved in assembly of mitochondrial respiratory complexes; may act as a receptor for proteins destined for export from the mitochondrial matrix to the inner membrane ORF Verified viable YBR186W S000000390 PCH2 Nucleolar component of the pachytene checkpoint, which prevents chromosome segregation when recombination and chromosome synapsis are defective; also represses meiotic interhomolog recombination in the rDNA ORF Verified viable YBR187W S000000391 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YBR187W is an essential gene ORF Uncharacterized viable YBR188C S000000392 NTC20 Member of a complex, including Prp19p, that binds to the spliceosome; required for pre-mRNA splicing ORF Verified viable YBR189W S000000393 RPS9B Protein component of the small (40S) ribosomal subunit; nearly identical to Rps9Ap and has similarity to E. coli S4 and rat S9 ribosomal proteins ORF Verified viable YBR191W S000000395 RPL21A Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl21Bp and has similarity to rat L21 ribosomal protein ORF Verified viable YBR194W S000000398 SOY1 Protein proposed to be associated with the nuclear pore complex ORF Uncharacterized viable YBR195C S000000399 MSI1 Subunit of chromatin assembly factor I (CAF-I), regulates the RAS/cAMP pathway via sequestration of Npr1p kinase; localizes to the nucleus and cytoplasm; homologous to human retinoblastoma binding proteins RbAp48 and RbAp46 ORF Verified viable YBR197C S000000401 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene ORF Uncharacterized viable YBR199W S000000403 KTR4 Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family ORF Verified viable YBR200W S000000404 BEM1 Protein containing SH3-domains, involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p ORF Verified viable YBR201W S000000405 DER1 Endoplasmic reticulum membrane protein, required for ER-associated protein degradation, involved in the retrograde transport of misfolded or unassembled proteins; N- and C- termini protrude into the cytoplasm, has similarity to Dfm1p ORF Verified viable YBR203W S000000407 COS111 Protein required for wild-type resistance to the antifungal drug ciclopirox olamine; not related to the COS family of subtelomerically-encoded proteins ORF Verified viable YBR204C S000000408 Serine hydrolase; YBR204C is not an essential gene ORF Uncharacterized viable YBR205W S000000409 KTR3 Putative alpha-1,2-mannosyltransferase involved in O- and N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family ORF Verified viable YBR206W S000000410 Hypothetical protein ORF Dubious viable YBR207W S000000411 FTH1 Putative high affinity iron transporter involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis ORF Verified viable YBR208C S000000412 DUR1,2 Urea amidolyase, contains both urea carboxylase and allophanate hydrolase activities, degrades urea to CO2 and NH3; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation ORF Verified viable YBR209W S000000413 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR209W is not an essential gene ORF Dubious viable YBR210W S000000414 ERV15 Protein of unknown function, has similarity to Erv14p ORF Verified viable YBR212W S000000416 NGR1 RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; expressed in stationary phase ORF Verified viable YBR213W S000000417 MET8 Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme; also involved in the expression of PAPS reductase and sulfite reductase ORF Verified viable YBR214W S000000418 SDS24 One of two S. cerevisiae homologs (Sds23p and Sds24p) of the Schizosaccharomyces pombe Sds23 protein, which genetic studies have implicated in APC/cyclosome regulation; may play an indirect role in fluid-phase endocytosis ORF Verified viable YBR215W S000000419 HPC2 Highly charged, basic protein required for normal cell-cycle regulation of histone gene transcription; mutants display strong synthetic defects with subunits of FACT, a complex that allows RNA Pol II to elongate through nucleosomes ORF Verified viable YBR216C S000000420 YBP1 Protein required for oxidation of specific cysteine residues of the transcription factor Yap1p, resulting in the nuclear localization of Yap1p in response to stress ORF Verified viable YBR217W S000000421 ATG12 Ubiquitin-like modifier, conjugated via an isopeptide bond to a lysine residue of Atg5p by the E1 enzyme, Atg7p, and the E2 enzyme, Atg10p, a step that is essential for autophagy ORF Verified viable YBR218C S000000422 PYC2 Pyruvate carboxylase isoform, cytoplasmic enzyme that converts pyruvate to oxaloacetate; highly similar to isoform Pyc1p but differentially regulated; mutations in the human homolog are associated with lactic acidosis ORF Verified viable YBR219C S000000423 Putative protein of unknown function; YBR219C is not an essential gene ORF Uncharacterized viable YBR220C S000000424 Putative protein of unknown function; YBR220C is not an essential gene ORF Uncharacterized viable YBR221C S000000425 PDB1 E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria ORF Verified viable YBR222C S000000426 PCS60 Peroxisomal AMP-binding protein, localizes to both the peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid, similar to E. coli long chain acyl-CoA synthetase ORF Verified viable YBR223C S000000427 TDP1 Tyrosyl-DNA Phosphodiesterase I, hydrolyzes 3'-phosphotyrosyl bonds to generate 3'-phosphate DNA and tyrosine, involved in the repair of DNA lesions created by topoisomerase I ORF Verified viable YBR224W S000000428 Hypothetical protein ORF Dubious viable YBR225W S000000429 Hypothetical protein ORF Uncharacterized viable YBR226C S000000430 Hypothetical protein ORF Dubious viable YBR227C S000000431 MCX1 Mitochondrial ATP-binding protein, possibly a mitochondrial chaperone with non-proteolytic function; similar to bacterial ClpX proteins ORF Verified viable YBR228W S000000432 SLX1 Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p ORF Verified viable YBR229C S000000433 ROT2 Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations ORF Verified viable YBR230C S000000434 OM14 Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron ORF Verified viable YBR231C S000000435 SWC5 Protein of unknown function, component of the Swr1p complex that incorporates Htz1p into chromatin ORF Uncharacterized viable YBR232C S000000436 Hypothetical protein ORF Dubious viable YBR233W S000000437 PBP2 RNA binding protein with similarity to mammalian heterogeneous nuclear RNP K protein, involved in the regulation of telomere position effect and telomere length ORF Verified viable YBR235W S000000439 Putative ion transporter, similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family; YBR235W is not an essential gene ORF Uncharacterized viable YBR238C S000000442 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and mitochondrion; YBR238C is not an essential gene; transcriptionally up-regulated by TOR and deletion increases life span ORF Uncharacterized viable YBR239C S000000443 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR239C is not an essential gene ORF Uncharacterized viable YBR240C S000000444 THI2 Zinc finger protein of the Zn(II)2Cys6 type, probable transcriptional activator of thiamine biosynthetic genes ORF Verified viable YBR241C S000000445 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane; YBR241C is not an essential gene ORF Uncharacterized viable YBR242W S000000446 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene ORF Uncharacterized viable YBR244W S000000448 GPX2 Phospholipid hydroperoxide glutathione peroxidase induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress ORF Verified viable YBR245C S000000449 ISW1 Member of the imitation-switch (ISWI) class of ATP-dependent chromatin remodeling complexes; ATPase that forms a complex with Ioc2p and Ioc4p to regulate transcription elongation, and a complex with Ioc3p to repress transcription initiation ORF Verified viable YBR246W S000000450 Hypothetical protein ORF Uncharacterized viable YBR248C S000000452 HIS7 Imidazole glycerol phosphate synthase (glutamine amidotransferase:cyclase), catalyzes the fifth and sixth steps of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor ORF Verified viable YBR249C S000000453 ARO4 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan ORF Verified viable YBR250W S000000454 Putative protein of unknown function; YBR250W is not essential gene ORF Uncharacterized viable YBR251W S000000455 MRPS5 Mitochondrial ribosomal protein of the small subunit ORF Verified viable YBR255W S000000459 Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern ORF Uncharacterized viable YBR258C S000000462 SHG1 Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres ORF Verified viable YBR259W S000000463 Putative protein of unknown function; YBR259W is not an essential gene ORF Uncharacterized viable YBR260C S000000464 RGD1 GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization ORF Verified viable YBR261C S000000465 Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YBR261C is not an essential gene ORF Uncharacterized viable YBR262C S000000466 FMP51 The authentic, non-tagged protein was localized to the mitochondria ORF Uncharacterized viable YBR263W S000000467 SHM1 Mitochondrial serine hydroxymethyltransferase, involved in one-carbon metabolism ORF Verified viable YBR264C S000000468 YPT10 GTP binding protein that contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles ORF Verified viable YBR266C S000000470 SLM6 Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase ORF Dubious viable YBR267W S000000471 REI1 Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network ORF Verified viable YBR268W S000000472 MRPL37 Mitochondrial ribosomal protein of the large subunit ORF Verified viable YBR269C S000000473 FMP21 The authentic, non-tagged protein was localized to the mitochondria ORF Uncharacterized viable YBR270C S000000474 BIT2 Hypothetical protein ORF Uncharacterized viable YBR271W S000000475 Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YBR271W is not as essential gene ORF Uncharacterized viable YBR272C S000000476 HSM3 Protein of unknown function, involved in DNA mismatch repair during slow growth; has weak similarity to Msh1p ORF Verified viable YBR273C S000000477 UBX7 UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p ORF Uncharacterized viable YBR274W S000000478 CHK1 DNA damage checkpoint effector kinase, mediates mitotic cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer kinase Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase ORF Verified viable YBR275C S000000479 RIF1 Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation ORF Verified viable YBR276C S000000480 PPS1 Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle ORF Verified viable YBR277C S000000481 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W ORF Dubious viable YBR278W S000000482 DPB3 Third-largest subunit of DNA polymerase II (DNA polymerase epsilon), required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; mRNA abundance peaks at the G1/S boundary of the cell cycle ORF Verified viable YBR279W S000000483 PAF1 RNA polymerase II-associated protein, defines a large complex that is biochemically and functionally distinct from the Srb-Mediator form of Pol II holoenzyme and is required for full expression of a subset of cell cycle-regulated genes ORF Verified viable YBR280C S000000484 Putative F-Box protein; interacts with Skp1; YBR280C is not an essential gene ORF Uncharacterized viable YBR281C S000000485 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR281C is not an essential gene ORF Uncharacterized viable YBR282W S000000486 MRPL27 Mitochondrial ribosomal protein of the large subunit ORF Verified viable YBR283C S000000487 SSH1 Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential ORF Verified viable YBR284W S000000488 Putative protein of unknown function; YBR284W is not an essential gene; null mutant exhibits decreased resistance to rapamycin and wortmannin ORF Uncharacterized viable YBR285W S000000489 Putative protein of unknown function; YBR287W is not an essential gene and deletion of the YBR287W leads to poor growth on glucose-minimal medium at 15C ORF Uncharacterized viable YBR286W S000000490 APE3 Vacuolar aminopeptidase Y, processed to mature form by Prb1p ORF Verified viable YBR287W S000000491 ZSP1 Protein of unknown function; mutation results in a zinc sensitive phenotype ORF Uncharacterized viable YBR288C S000000492 APM3 Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway ORF Verified viable YBR289W S000000493 SNF5 Subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p ORF Verified viable YBR290W S000000494 BSD2 Heavy metal ion homeostasis protein, facilitates trafficking of Smf1p and Smf2p metal transporters to the vacuole where they are degraded, controls metal ion transport, prevents metal hyperaccumulation, functions in copper detoxification ORF Verified viable YBR291C S000000495 CTP1 Mitochondrial inner membrane citrate transporter, member of the mitochondrial carrier family ORF Verified viable YBR292C S000000496 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR292C is not an essential gene ORF Dubious viable YBR293W S000000497 VBA2 Permease of basic amino acids in the vacuolar membrane ORF Verified viable YBR294W S000000498 SUL1 High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates ORF Verified viable YBR295W S000000499 PCA1 P-type metal-transporting ATPase with a role in copper and iron homeostasis; R970G-substitution in the C-terminal region confers cadmium resistance ORF Verified viable YBR296C S000000500 PHO89 Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p ORF Verified viable YBR297W S000000501 MAL33 MAL-activator protein, part of complex locus MAL3; nonfunctional in genomic reference strain S288C ORF Verified viable YBR298C S000000502 MAL31 Maltose permease, high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL3 complex locus; member of the 12 transmembrane domain superfamily of sugar transporters; functional in genomic reference strain S288C ORF Verified viable YBR300C S000000504 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene ORF Dubious viable YBR301W S000000505 DAN3 Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth ORF Verified viable YCL001W S000000507 RER1 Protein involved in retention of membrane proteins, including Sec12p, in the ER; localized to Golgi; functions as a retrieval receptor in returning membrane proteins to the ER ORF Verified viable YCL001W-A S000007221 Putative protein of unknown function; YCL001W-A is not an essential gene ORF Uncharacterized viable YCL002C S000000508 Putative protein of unknown function; YCL002C is not an essential gene ORF Uncharacterized viable YCL005W S000000511 LDB16 Protein of unknown function; null mutant shows a reduced affinity for the alcian blue dye suggesting a decreased net negative charge of the cell surface ORF Verified viable YCL007C S000000513 Dubious ORF unlikely to encode a protein; overlaps verified ORF YCL005W-A; mutations in YCL007C were thought to confer sensitivity to calcofluor white, but this phenotype was later shown to be due to the defect in YCL005W-A ORF Dubious viable YCL008C S000000514 STP22 Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype ORF Verified viable YCL009C S000000515 ILV6 Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria ORF Verified viable YCL010C S000000516 SGF29 SaGa associated Factor 29kDa; Probable 29kKDa Subunit of SAGA histone acetyltransferase complex ORF Verified viable YCL011C S000000517 GBP2 Poly(A+) RNA-binding protein, involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Hrb1p and Npl3p; also binds single-stranded telomeric repeat sequence in vitro ORF Verified viable YCL014W S000000520 BUD3 Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding ORF Verified viable YCL016C S000000521 DCC1 Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance ORF Verified viable YCL022C S000000527 Hypothetical protein ORF Dubious viable YCL023C S000000528 Hypothetical protein ORF Dubious viable YCL024W S000000529 KCC4 Protein kinase of the bud neck involved in the septin checkpoint, associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p ORF Verified viable YCL025C S000000530 AGP1 Low-affinity amino acid permease with broad substrate range, involved in uptake of asparagine, glutamine, and other amino acids; expression is regulated by the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p) ORF Verified viable YCL026C-A S000000589 FRM2 Protein of unknown function, involved in the integration of lipid signaling pathways with cellular homeostasis ORF Verified viable YCL027W S000000532 FUS1 Membrane protein localized to the shmoo tip, required for cell fusion; expression regulated by mating pheromone; proposed to coordinate signaling, fusion, and polarization events required for fusion; potential Cdc28p substrate ORF Verified viable YCL028W S000000533 RNQ1 [PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate ORF Verified viable YCL029C S000000534 BIK1 Microtubule-associated protein, component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170 ORF Verified viable YCL030C S000000535 HIS4 Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase, phosphoribosyl-AMP cyclohydrolase, and histidinol dehydrogenase activities; catalyzes the second, third, ninth and tenth steps in histidine biosynthesis ORF Verified viable YCL032W S000000537 STE50 Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction ORF Verified viable YCL033C S000000538 Putative protein-methionine-R-oxide reductase; involved in response to oxidative stress; similar to mouse Sepx1p and fly SelRp; YCL033C is not an essential gene ORF Uncharacterized viable YCL034W S000000539 LSB5 Protein of unknown function; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization ORF Verified viable YCL035C S000000540 GRX1 Hydroperoxide and superoxide-radical responsive heat-stable glutathione-dependent disulfide oxidoreductase with active site cysteine pair; protects cells from oxidative damage ORF Verified viable YCL036W S000000541 GFD2 Protein of unknown function, identified as a high-copy suppressor of a dbp5 mutation ORF Uncharacterized viable YCL037C S000000542 SRO9 Cytoplasmic RNA-binding protein that associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif ORF Verified viable YCL038C S000000543 ATG22 Protein required for the breakdown of autophagic vesicles in the vacuole during autophagy, putative integral membrane protein that localizes to vacuolar membranes and punctate structures attached to the vacuole ORF Verified viable YCL039W S000000544 GID7 Protein of unknown function, involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions ORF Verified viable YCL040W S000000545 GLK1 Glucokinase, catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources ORF Verified viable YCL042W S000000547 Hypothetical protein ORF Uncharacterized viable YCL044C S000000549 MGR1 Subunit, with Yme1p, of the mitochondrial inner membrane i-AAA protease complex, which is responsible for degradation of unfolded or misfolded mitochondrial gene products; required for growth of cells lacking the mitochondrial genome ORF Verified viable YCL045C S000000550 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; interacts with Gal80p; YCL045C is not an essential gene ORF Uncharacterized viable YCL046W S000000551 Hypothetical protein ORF Dubious viable YCL047C S000000552 Hypothetical protein ORF Uncharacterized viable YCL048W S000000553 SPS22 Protein of unknown function, redundant with Sps2p for the organization of the beta-glucan layer of the spore wall ORF Verified viable YCL049C S000000554 Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene; ORF Uncharacterized viable YCL050C S000000555 APA1 Diadenosine 5',5''-P1,P4-tetraphosphate phosphorylase I (AP4A phosphorylase), involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; has similarity to Apa2p ORF Verified viable YCL051W S000000556 LRE1 Protein involved in control of cell wall structure and stress response; inhibits Cbk1p protein kinase activity; overproduction confers resistance to cell-wall degrading enzymes ORF Verified viable YCL055W S000000560 KAR4 Transcription factor required for induction of KAR3 and CIK1 during mating, also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone ORF Verified viable YCL056C S000000561 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern ORF Uncharacterized viable YCL057W S000000562 PRD1 Zinc metalloendopeptidase, found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins ORF Verified viable YCL058C S000000563 FYV5 Protein of unknown function, required for survival upon exposure to K1 killer toxin; involved in ion homeostasis ORF Dubious viable YCL061C S000000566 MRC1 S-phase checkpoint protein found at replication forks, required for DNA replication; also required for Rad53p activation during DNA replication stress, where it forms a replication-pausing complex with Tof1p and is phosphorylated by Mec1p; protein involved in replication checkpoint ORF Verified viable YCL063W S000000568 VAC17 Protein involved in vacuole inheritance; acts as a vacuole-specific receptor for myosin Myo2p ORF Verified viable YCL064C S000000569 CHA1 Catabolic L-serine (L-threonine) deaminase, catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine ORF Verified viable YCL069W S000000574 VBA3 Permease of basic amino acids in the vacuolar membrane ORF Verified viable YCL076W S000000581 Hypothetical protein ORF Dubious viable YCR001W S000000594 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR001W is not an essential gene ORF Dubious viable YCR002C S000000595 CDC10 Component of the septin ring of the mother-bud neck that is required for cytokinesis; septins recruit proteins to the neck and can act as a barrier to diffusion at the membrane, and they comprise the 10nm filaments seen with EM ORF Verified viable YCR003W S000000596 MRPL32 Mitochondrial ribosomal protein of the large subunit ORF Verified viable YCR004C S000000597 YCP4 Protein of unknown function, has sequence and structural similarity to flavodoxins; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern ORF Verified viable YCR005C S000000598 CIT2 Citrate synthase, catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate, peroxisomal isozyme involved in glyoxylate cycle; expression is controlled by Rtg1p and Rtg2p transcription factors ORF Verified viable YCR006C S000000599 Hypothetical protein ORF Dubious viable YCR007C S000000600 Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene ORF Uncharacterized viable YCR008W S000000601 SAT4 Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p ORF Verified viable YCR009C S000000602 RVS161 Amphiphysin-like lipid raft protein; subunit of a complex (Rvs161p-Rvs167p) that regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress ORF Verified viable YCR010C S000000603 ADY2 Acetate transporter required for normal sporulation ORF Verified viable YCR011C S000000604 ADP1 Putative ATP-dependent permease of the ABC transporter family of proteins ORF Verified viable YCR014C S000000607 POL4 DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta ORF Verified viable YCR015C S000000608 Putative protein of unknown function; YCR015C is not an essential gene ORF Uncharacterized viable YCR016W S000000609 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; YCR016W is not an essential gene ORF Uncharacterized viable YCR017C S000000610 CWH43 Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion ORF Verified viable YCR019W S000000612 MAK32 Protein necessary for structural stability of L-A double-stranded RNA-containing particles ORF Verified viable YCR020C S000000613 PET18 Protein required for respiratory growth and stability of the mitochondrial genome ORF Verified viable YCR020C-A S000000614 MAK31 Non-catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus; member of the Sm protein family ORF Verified viable YCR020W-B S000006439 HTL1 Subunit of the RSC chromatin remodeling complex, a multisubunit complex that functions in transcriptional regulation, chromosome stability and establishing sister chromatid cohesion; involved in telomere maintenance ORF Verified viable YCR021C S000000615 HSP30 Hydrophobic plasma membrane localized, stress-responsive protein that negatively regulates the H(+)-ATPase Pma1p; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase ORF Verified viable YCR022C S000000616 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR022C is not an essential gene ORF Dubious viable YCR023C S000000617 Putative protein of unknown function; YCR023C is not an essential gene ORF Uncharacterized viable YCR024C S000000618 SLM5 Mitochondrial asparaginyl-tRNA synthetase ORF Verified viable YCR024C-A S000000619 PMP1 Small single-membrane span proteolipid that functions as a regulatory subunit of the plasma membrane H(+)-ATPase Pma1p, forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines ORF Verified viable YCR025C S000000620 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene ORF Dubious viable YCR026C S000000621 NPP1 Nucleotide pyrophosphatase/phosphodiesterase family member; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation ORF Verified viable YCR027C S000000622 RHB1 Putative Rheb-related GTPase involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins ORF Verified viable YCR028C S000000623 FEN2 Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph ORF Verified viable YCR028C-A S000007222 RIM1 Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication ORF Verified viable YCR030C S000000626 SYP1 Protein with a potential role in actin cytoskeletal organization; overexpression suppresses a pfy1 (profilin) null mutation ORF Verified viable YCR031C S000000627 RPS14A Ribosomal protein 59 of the small subunit, required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; nearly identical to Rps14Bp and similar to E. coli S11 and rat S14 ribosomal proteins ORF Verified viable YCR032W S000000628 BPH1 Protein homologous to human Chediak-Higashi syndrome protein and murine beige gene, which are implicated in disease syndromes due to defective lysosomal trafficking ORF Verified viable YCR033W S000000629 SNT1 Subunit of the Set3C deacetylase complex; putative DNA-binding protein ORF Verified viable YCR034W S000000630 FEN1 Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway ORF Verified viable YCR036W S000000632 RBK1 Putative ribokinase ORF Verified viable YCR037C S000000633 PHO87 Low-affinity inorganic phosphate (Pi) transporter, involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments ORF Verified viable YCR043C S000000639 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene ORF Uncharacterized viable YCR044C S000000640 PER1 Vacuolar membrane protein that seems to be involved in Mn2+ homeostasis; mutant is dependent on activation of the unfolded protein response pathway for viability ORF Verified viable YCR045C S000000641 Hypothetical protein ORF Uncharacterized viable YCR046C S000000642 IMG1 Mitochondrial ribosomal protein of the small subunit, required for respiration and for maintenance of the mitochondrial genome ORF Verified viable YCR047C S000000643 BUD23 Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern ORF Verified viable YCR048W S000000644 ARE1 Acyl-CoA:sterol acyltransferase, isozyme of Are2p; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the absence of oxygen ORF Verified viable YCR049C S000000645 Hypothetical protein ORF Dubious viable YCR050C S000000646 Hypothetical protein ORF Dubious viable YCR051W S000000647 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene ORF Uncharacterized viable YCR053W S000000649 THR4 Threonine synthase, conserved protein that catalyzes formation of threonine from 0-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway ORF Verified viable YCR059C S000000655 YIH1 Protein that inhibits activation of Gcn2p, an eIF2 alpha subunit protein kinase, by competing for Gcn1p binding, thus impacting gene expression in response to starvation; has sequence and functional similarity to the mouse IMPACT gene ORF Verified viable YCR060W S000000656 TAH1 HSP90 cofactor; interacts with Hsp82p, Pih1p, Rvb1 and Rvb2, contains a single TPR domain with at least two TPR motifs ORF Verified viable YCR061W S000000657 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern ORF Uncharacterized viable YCR063W S000000659 BUD31 Protein involved in bud-site selection; analysis of integrated high-throughput datasets predicts an involvement in RNA splicing; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern ORF Verified viable YCR064C S000000660 Hypothetical protein ORF Dubious viable YCR065W S000000661 HCM1 Forkhead transcription factor that drives S-phase specific expression of genes involved in chromosome segregation, spindle dynamics, and budding; suppressor of calmodulin mutants with specific SPB assembly defects; telomere maintenance role ORF Verified viable YCR066W S000000662 RAD18 Protein involved in postreplication repair; binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; forms heterodimer with Rad6p; contains RING-finger motif ORF Verified viable YCR067C S000000663 SED4 Integral endoplasmic reticulum membrane protein, functions as a positive regulator of Sar1p probably through inhibition of GTPase activation by Sec23p; binds Sec16p, participates in vesicle formation, similar to Sec12p ORF Verified viable YCR068W S000000664 ATG15 Lipase, required for intravacuolar lysis of autophagic bodies; located in the endoplasmic reticulum membrane and targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway ORF Verified viable YCR069W S000000665 CPR4 Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway ORF Verified viable YCR071C S000000667 IMG2 Mitochondrial ribosomal protein of the small subunit ORF Verified viable YCR073C S000000669 SSK22 MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; functionally redundant with, and homologous to, Ssk2p; interacts with and is activated by Ssk1p; phosphorylates Pbs2p ORF Verified viable YCR073W-A S000000718 SOL2 Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol1p, Sol3p, and Sol4p ORF Verified viable YCR075C S000000671 ERS1 Protein with similarity to human cystinosin, which is a H(+)-driven transporter involved in L-cystine export from lysosomes and implicated in the disease cystinosis; contains seven transmembrane domains ORF Verified viable YCR076C S000000672 Putative protein of unknown function; YCR076C is not an essential gene ORF Uncharacterized viable YCR077C S000000673 PAT1 Topoisomerase II-associated deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; functionally linked to Pab1p ORF Verified viable YCR079W S000002133 AUP1 Putative phosphoprotein phosphatase; contains mitochondrial targeting sequence; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; YCR079W is not an essential gene ORF Uncharacterized viable YCR081W S000000677 SRB8 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression ORF Verified viable YCR082W S000000678 AHC2 Protein of unknown function, putative transcriptional regulator; proposed to be a Ada Histone acetyltransferase complex component; GFP tagged protein is localized to the cytoplasm and nucleus ORF Uncharacterized viable YCR083W S000000679 TRX3 Mitochondrial thioredoxin, highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p ORF Verified viable YCR084C S000000680 TUP1 General repressor of transcription, forms complex with Cyc8p, involved in the establishment of repressive chromatin structure through interactions with histones H3 and H4, appears to enhance expression of some genes ORF Verified viable YCR085W S000000681 Hypothetical protein ORF Dubious viable YCR086W S000000682 CSM1 Nucleolar protein that forms a complex with Lrs4p which binds Mam1p at kinetochores during meiosis I to mediate accurate chromosome segregation, may be involved in premeiotic DNA replication; possible role in telomere maintenance ORF Verified viable YCR087C-A S000007223 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene ORF Uncharacterized viable YCR087W S000000683 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene ORF Dubious viable YCR088W S000000684 ABP1 Actin-binding protein of the cortical actin cytoskeleton, important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization ORF Verified viable YCR089W S000000685 FIG2 Cell wall adhesin, expressed specifically during mating; may be involved in maintenance of cell wall integrity during mating ORF Verified viable YCR090C S000000686 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene ORF Uncharacterized viable YCR091W S000000687 KIN82 Putative serine/threonine protein kinase, most similar to cyclic nucleotide-dependent protein kinase subfamily and the protein kinase C subfamily ORF Verified viable YCR092C S000000688 MSH3 Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability ORF Verified viable YCR094W S000000690 CDC50 Endosomal protein that regulates cell polarity; similar to Ynr048wp and Lem3p ORF Verified viable YCR095C S000000691 OCA4 Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts ORF Uncharacterized viable YCR098C S000000695 GIT1 Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability ORF Verified viable YCR099C S000000696 Hypothetical protein ORF Uncharacterized viable YCR100C S000000697 Hypothetical protein ORF Uncharacterized viable YCR101C S000000698 Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene ORF Uncharacterized viable YCR102C S000000699 Putative protein of unknown function; involved in copper metabolism; similar to C.carbonum toxD gene; YCR102C is not an essential gene ORF Uncharacterized viable YCR102W-A S000007231 Similar to several yeast probable membrane proteins, including YNR075W and YFL062W ORF Dubious viable YCR105W S000000702 ADH7 NADPH-dependent cinnamyl alcohol dehydrogenase family member with broad substrate specificity; may be involved in fusel alcohol synthesis ORF Verified viable YCR106W S000000703 RDS1 Zinc cluster protein involved in conferring resistance to cycloheximide ORF Verified viable YDL001W S000002159 RMD1 Cytoplasmic protein required for sporulation ORF Verified viable YDL002C S000002160 NHP10 Protein related to mammalian high mobility group proteins; likely component of the INO80 complex, which is an ATP-dependent chromatin-remodeling complex ORF Verified viable YDL005C S000002163 MED2 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation ORF Verified viable YDL006W S000002164 PTC1 Type 2C protein phosphatase (PP2C); inactivates the osmosensing MAPK cascade by dephosphorylating Hog1p; mutation delays mitochondrial inheritance; deletion reveals defects in precursor tRNA splicing, sporulation and cell separation ORF Verified viable YDL009C S000002167 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YDL010W; YDL009C is not an essential gene ORF Dubious viable YDL010W S000002168 Putative thiol-disulfide oxidoreductase; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YDL010W is not an essential gene ORF Uncharacterized viable YDL011C S000002169 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YDL010W ORF Dubious viable YDL012C S000002170 Plasma membrane protein of unknown function; YDL012C is not an essential gene ORF Uncharacterized viable YDL013W S000002171 HEX3 Protein containing a RING finger domain that interacts with Slx8p; mutant phenotypes and genetic interactions suggest a role in sumoylation and in genome stability ORF Verified viable YDL018C S000002176 ERP3 Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport ORF Verified viable YDL019C S000002177 OSH2 Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability ORF Verified viable YDL020C S000002178 RPN4 Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses ORF Verified viable YDL021W S000002179 GPM2 Homolog of Gpm1p phosphoglycerate mutase which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event ORF Verified viable YDL022W S000002180 GPD1 NAD-dependent glycerol-3-phosphate dehydrogenase, key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p ORF Verified viable YDL023C S000002181 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 80% of ORF overlaps the verified gene GPD1; deletion in cyr1 mutant results in loss of stress resistance ORF Dubious viable YDL024C S000002182 DIA3 Protein of unknown function, involved in invasive and pseudohyphal growth ORF Verified viable YDL025C S000002183 Putative protein kinase, potentially phosphorylated by Cdc28p; YDL025C is not an essential gene ORF Uncharacterized viable YDL026W S000002184 Hypothetical protein ORF Dubious viable YDL027C S000002185 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; YDL027C is not an essential gene ORF Uncharacterized viable YDL032W S000002190 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene SLM3/YDL033C; YDL032W is not an essential gene ORF Dubious viable YDL033C S000002191 SLM3 tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) ORF Uncharacterized viable YDL034W S000002192 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps with verified gene GPR1/YDL035C; YDL034W is not an essential gene ORF Dubious viable YDL035C S000002193 GPR1 Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis ORF Verified viable YDL036C S000002194 PUS9 Mitochondrial tRNA pseudouridine synthase involved in pseudouridylation of mitochondrial tRNAs at position 32 ORF Verified viable YDL037C S000002195 BSC1 Protein of unconfirmed function, similar to cell surface flocculin Muc1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression ORF Verified viable YDL038C S000002196 Putative protein of unknown function; YDL038C is not an essential gene ORF Uncharacterized viable YDL039C S000002197 PRM7 Pheromone-regulated protein, predicted to have one transmembrane segment; promoter contains Gcn4p binding elements ORF Verified viable YDL040C S000002198 NAT1 Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing ORF Verified viable YDL041W S000002199 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C ORF Dubious viable YDL042C S000002200 SIR2 Conserved NAD+ dependent histone deacetylase of the Sirtuin family involved in regulation of lifespan; plays roles in silencing at HML, HMR, telomeres, and the rDNA locus; negatively regulates initiation of DNA replication ORF Verified viable YDL044C S000002202 MTF2 Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription ORF Verified viable YDL045W-A S000006430 MRP10 Mitochondrial ribosomal protein of the small subunit ORF Verified viable YDL046W S000002204 NPC2 Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes ORF Verified viable YDL047W S000002205 SIT4 Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization ORF Verified viable YDL048C S000002206 STP4 Protein containing a Kruppel-type zinc-finger domain; has similarity to Stp1p, Stp2p, and Stp3p ORF Verified viable YDL049C S000002207 KNH1 Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant ORF Verified viable YDL050C S000002208 Hypothetical protein ORF Dubious viable YDL051W S000002209 LHP1 RNA binding protein required for maturation of tRNA and snRNA precursors; acts as a molecular chaperone for RNAs transcribed by polymerase III; homologous to human La (SS-B) autoantigen ORF Verified viable YDL052C S000002210 SLC1 1-acyl-sn-gylcerol-3-phosphate acyltransferase, catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; located in lipid particles and endoplasmic reticulum ORF Verified viable YDL053C S000002211 PBP4 Pbp1p binding protein, interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay ORF Verified viable YDL054C S000002212 MCH1 Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport ORF Verified viable YDL056W S000002214 MBP1 Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes ORF Verified viable YDL057W S000002215 Putative protein of unknown function; YDL057W is not an essential gene ORF Uncharacterized viable YDL059C S000002217 RAD59 Protein involved in the repair of double-strand breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; homologous to Rad52p ORF Verified viable YDL061C S000002219 RPS29B Protein component of the small (40S) ribosomal subunit; nearly identical to Rps29Ap and has similarity to rat S29 and E. coli S14 ribosomal proteins ORF Verified viable YDL062W S000002220 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YDL063C; YDL062W is not essential ORF Dubious viable YDL063C S000002221 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL063C is not an essential gene ORF Uncharacterized viable YDL065C S000002223 PEX19 Chaperone and import receptor for newly-synthesized class I peroxisomal membrane proteins (PMPs), binds PMPs in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane ORF Verified viable YDL066W S000002224 IDP1 Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes ORF Verified viable YDL067C S000002225 COX9 Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain ORF Verified viable YDL068W S000002226 Hypothetical protein ORF Dubious viable YDL069C S000002227 CBS1 Mitochondrial translational activator of the COB mRNA; membrane protein that interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader ORF Verified viable YDL070W S000002228 BDF2 Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p ORF Verified viable YDL071C S000002229 IRC1 Hypothetical protein; null mutant displays increased levels of spontaneous Rad52 foci ORF Dubious viable YDL072C S000002230 YET3 Endoplasmic reticulum transmembrane protein, homolog of human BAP31 protein ORF Uncharacterized viable YDL073W S000002231 Putative protein of unknown function; YDL073W is not an essential gene ORF Uncharacterized viable YDL074C S000002232 BRE1 E3 ubiquitin ligase for Rad6p, required for the ubiquitination of histone H2B, recruitment of Rad6p to promoter chromatin and subsequent methylation of histone H3 (on L4 and L79), contains RING finger domain ORF Verified viable YDL075W S000002233 RPL31A Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl31Bp and has similarity to rat L31 ribosomal protein; associates with the karyopherin Sxm1p ORF Verified viable YDL076C S000002234 RXT3 Protein of unknown function, may be involved in chromatin silencing ORF Uncharacterized viable YDL077C S000002235 VAM6 Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p ORF Verified viable YDL078C S000002236 MDH3 Cytoplasmic malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the glyoxylate cycle ORF Verified viable YDL079C S000002237 MRK1 Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation ORF Verified viable YDL080C S000002238 THI3 Probable decarboxylase, required for expression of enzymes involved in thiamine biosynthesis; may have a role in catabolism of amino acids to long-chain and complex alcohols ORF Verified viable YDL081C S000002239 RPP1A Ribosomal protein P1 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component ORF Verified viable YDL082W S000002240 RPL13A Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl13Bp; not essential for viability; has similarity to rat L13 ribosomal protein ORF Verified viable YDL083C S000002241 RPS16B Protein component of the small (40S) ribosomal subunit; identical to Rps16Ap and has similarity to E. coli S9 and rat S16 ribosomal proteins ORF Verified viable YDL085W S000002243 NDE2 Mitochondrial external NADH dehydrogenase, catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p are involved in providing the cytosolic NADH to the mitochondrial respiratory chain ORF Verified viable YDL086W S000002244 Putative protein of unknown function; localizes to the mitochondrion; YDL086W is not an essential gene ORF Uncharacterized viable YDL088C S000002246 ASM4 Nuclear pore complex subunit, part of a subcomplex also containing Nup53p, Nup170p, and Pse1p ORF Verified viable YDL089W S000002247 Protein of unknown function; interacts with meiotic division protein Csm1p; green fluorescent protein (GFP)-fusion protein localizes to the nuclear periphery, potential Cdc28p substrate ORF Uncharacterized viable YDL090C S000002248 RAM1 Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit ORF Verified viable YDL091C S000002249 UBX3 UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern ORF Uncharacterized viable YDL093W S000002251 PMT5 Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt3p, can instead interact with Pmt2p in some conditions; target for new antifungals ORF Verified viable YDL094C S000002252 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verfied gene PMT5/YDL093W; YDL094C is not essential ORF Dubious viable YDL095W S000002253 PMT1 Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt2p, can instead interact with Pmt3p in some conditions; target for new antifungals ORF Verified viable YDL096C S000002254 OPI6 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential ORF Dubious viable YDL099W S000002257 BUG1 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern ORF Uncharacterized viable YDL100C S000002258 GET3 ATPase, subunit of the GET complex; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion; involved in resistance to heat and metal stress ORF Verified viable YDL101C S000002259 DUN1 Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair ORF Verified viable YDL104C S000002262 QRI7 Putative metalloprotease, similar to O-sialoglycoprotein metallopeptidase from P. haemolytica ORF Uncharacterized viable YDL107W S000002265 MSS2 Peripherally bound inner membrane protein of the mitochondrial matrix, required for export of C-terminal tail of Cox2p through the inner membrane ORF Verified viable YDL109C S000002267 Putative lipase; involved in lipid metabolism; YDL109C is not an essential gene ORF Uncharacterized viable YDL110C S000002268 TMA17 Protein of unknown function that associates with ribosomes ORF Verified viable YDL112W S000002270 TRM3 2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs ORF Verified viable YDL113C S000002271 ATG20 Protein required for transport of aminopeptidase I (Lap4p) through the cytoplasm-to-vacuole targeting pathway; binds phosphatidylinositol-3-phosphate, involved in localization of membranes to the preautophagosome, potential Cdc28p substrate ORF Verified viable YDL114W S000002272 Putative protein of unknown function with similarity to acyl-carrier-protein reductases; YDL114W is not an essential gene ORF Uncharacterized viable YDL115C S000002273 IWR1 Protein of unknown function, deletion causes hypersensitivity to the K1 killer toxin ORF Uncharacterized viable YDL116W S000002274 NUP84 Subunit of the nuclear pore complex (NPC), forms a subcomplex with Nup85p, Nup120p, Nup145p-C, Sec13p, and Seh1p that plays a role in nuclear mRNA export and NPC biogenesis ORF Verified viable YDL117W S000002275 CYK3 SH3-domain protein located in the mother-bud neck and the cytokinetic actin ring; mutant phenotype and genetic interactions suggest a role in cytokinesis ORF Verified viable YDL118W S000002276 Hypothetical protein ORF Dubious viable YDL119C S000002277 Putative mitochondrial transport protein; localizes to the mitochondrion; YDL119C is not an essential gene ORF Uncharacterized viable YDL121C S000002279 Hypothetical protein ORF Uncharacterized viable YDL122W S000002280 UBP1 Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains ORF Verified viable YDL123W S000002281 SNA4 Protein of unknown function, localized to the vacuolar outer membrane ORF Verified viable YDL124W S000002282 NADPH-dependent alpha-keto amide reductase; reduces aromatic alpha-keto amides, aliphatic alpha-keto esters, and aromatic alpha-keto esters ORF Verified viable YDL125C S000002283 HNT1 Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint ORF Verified viable YDL127W S000002285 PCL2 G1 cyclin, associates with Pho85p cyclin-dependent kinase (Cdk) to contribute to entry into the mitotic cell cycle, essential for cell morphogenesis; localizes to sites of polarized cell growth ORF Verified viable YDL128W S000002286 VCX1 Vacuolar H+/Ca2+ exchanger involved in control of cytosolic Ca2+ concentration; has similarity to sodium/calcium exchangers, including the bovine Na+/Ca2+,K+ antiporter ORF Verified viable YDL129W S000002287 Hypothetical protein ORF Uncharacterized viable YDL130W S000002288 RPP1B Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component ORF Verified viable YDL130W-A S000007232 STF1 Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein ORF Verified viable YDL131W S000002289 LYS21 Homocitrate synthase isozyme, catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; highly similar to the other isozyme, Lys20p ORF Verified viable YDL133C-A S000002293 RPL41B Ribosomal protein L47 of the large (60S) ribosomal subunit, identical to Rpl41Ap and has similarity to rat L41 ribosomal protein; comprised of only 25 amino acids; rpl41a rpl41b double null mutant is viable ORF Verified viable YDL133W S000002291 Hypothetical protein ORF Uncharacterized viable YDL134C S000002292 PPH21 Catalytic subunit of protein phosphatase 2A, functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis ORF Verified viable YDL135C S000002294 RDI1 Rho GDP dissociation inhibitor involved in the localization and regulation of Cdc42p ORF Verified viable YDL136W S000002295 RPL35B Protein component of the large (60S) ribosomal subunit, identical to Rpl35Ap and has similarity to rat L35 ribosomal protein ORF Verified viable YDL137W S000002296 ARF2 ADP-ribosylation factor, GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; functionally interchangeable with Arf1p ORF Verified viable YDL138W S000002297 RGT2 Plasma membrane glucose receptor, highly similar to Snf3p; both Rgt2p and Snf3p serve as transmembrane glucose sensors generating an intracellular signal that induces expression of glucose transporter (HXT) genes ORF Verified viable YDL142C S000002301 CRD1 Cardiolipin synthase; produces cardiolipin, which is an important constituent of mitochondrial membranes; required for normal mitochondrial membrane potential and function ORF Verified viable YDL144C S000002303 Hypothetical protein ORF Uncharacterized viable YDL146W S000002305 LDB17 Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; null mutant shows a reduced affinity for the alcian blue dye suggesting a decreased net negative charge of the cell surface ORF Verified viable YDL149W S000002308 ATG9 Transmembrane protein involved in formation of Cvt and autophagic vesicles; cycles between the pre-autophagosomal structure and other cytosolic punctate structures, not found in autophagosomes ORF Verified viable YDL154W S000002313 MSH5 Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans ORF Verified viable YDL155W S000002314 CLB3 B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation ORF Verified viable YDL156W S000002315 Hypothetical protein ORF Uncharacterized viable YDL157C S000002316 Hypothetical protein ORF Uncharacterized viable YDL158C S000002317 Hypothetical protein ORF Dubious viable YDL159W S000002318 STE7 Signal transducing MAP kinase kinase involved in pheromone response, where it phosphorylates Fus3p, and in the pseudohyphal/invasive growth pathway, through phosphorylation of Kss1p; phosphorylated by Ste11p, degraded by ubiquitin pathway ORF Verified viable YDL160C S000002319 DHH1 Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping, coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation ORF Verified viable YDL161W S000002320 ENT1 Epsin-like protein involved in endocytosis and actin patch assembly and functionally redundant with Ent2p; binds clathrin via a clathrin-binding domain motif at C-terminus ORF Verified viable YDL162C S000002321 Hypothetical protein ORF Dubious viable YDL167C S000002326 NRP1 Protein of unknown function, rich in asparagine residues ORF Verified viable YDL168W S000002327 SFA1 Bifunctional enzyme containing both alcohol dehydrogenase and glutathione-dependent formaldehyde dehydrogenase activities, functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p ORF Verified viable YDL169C S000002328 UGX2 Protein of unknown function, transcript accumulates in response to any combination of stress conditions ORF Verified viable YDL170W S000002329 UGA3 Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus ORF Verified viable YDL171C S000002330 GLT1 NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source ORF Verified viable YDL172C S000002331 Hypothetical protein ORF Dubious viable YDL173W S000002332 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDL173W is not an essential gene ORF Uncharacterized viable YDL174C S000002333 DLD1 D-lactate dehydrogenase, oxidizes D-lactate to pyruvate, transcription is heme-dependent, repressed by glucose, and derepressed in ethanol or lactate; located in the mitochondrial inner membrane ORF Verified viable YDL175C S000002334 AIR2 RING finger protein that interacts with the arginine methyltransferase Hmt1p; may regulate methylation of Npl3p, which modulates Npl3p function in mRNA processing and export; has similarity to Air1p ORF Verified viable YDL176W S000002335 Hypothetical protein ORF Uncharacterized viable YDL177C S000002336 Hypothetical protein ORF Uncharacterized viable YDL178W S000002337 DLD2 D-lactate dehydrogenase, located in the mitochondrial matrix ORF Verified viable YDL179W S000002338 PCL9 Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p ORF Verified viable YDL180W S000002339 Hypothetical protein ORF Uncharacterized viable YDL181W S000002340 INH1 Protein that inhibits ATP hydrolysis by the F1F0-ATP synthase, inhibitory function is enhanced by stabilizing proteins Stf1p and Stf2p; has similarity to Stf1p and both Inh1p and Stf1p exhibit the potential to form coiled-coil structures ORF Verified viable YDL182W S000002341 LYS20 Homocitrate synthase isozyme, catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; highly similar to the other isozyme, Lys21p ORF Verified viable YDL183C S000002342 Putative protein of unknown function; YBR063C is not an essential gene ORF Uncharacterized viable YDL184C S000002343 RPL41A Ribosomal protein L47 of the large (60S) ribosomal subunit, identical to Rpl41Bp and has similarity to rat L41 ribosomal protein; comprised of only 25 amino acids; rpl41a rpl41b double null mutant is viable ORF Verified viable YDL185W S000002344 TFP1 Vacuolar ATPase V1 domain subunit A containing the catalytic nucleotide binding sites; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease ORF Verified viable YDL186W S000002345 Hypothetical protein ORF Uncharacterized viable YDL187C S000002346 Hypothetical protein ORF Dubious viable YDL188C S000002347 PPH22 Catalytic subunit of protein phosphatase 2A, functionally redundant with Pph21p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis ORF Verified viable YDL189W S000002348 RBS1 Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain ORF Verified viable YDL190C S000002349 UFD2 Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 ORF Verified viable YDL191W S000002350 RPL35A Protein component of the large (60S) ribosomal subunit, identical to Rpl35Bp and has similarity to rat L35 ribosomal protein ORF Verified viable YDL192W S000002351 ARF1 ADP-ribosylation factor, GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; functionally interchangeable with Arf2p ORF Verified viable YDL194W S000002353 SNF3 Plasma membrane glucose sensor that regulates glucose transport; has 12 predicted transmembrane segments; long cytoplasmic C-terminal tail is required for low glucose induction of hexose transporter genes HXT2 and HXT4 ORF Verified viable YDL197C S000002356 ASF2 Anti-silencing protein that causes derepression of silent loci when overexpressed ORF Verified viable YDL198C S000002357 GGC1 Mitochondrial GTP/GDP transporter, essential for mitochondrial genome maintenance; has a role in mitochondrial iron transport; member of the mitochondrial carrier family; (putative) mitochondrial carrier protein ORF Verified viable YDL199C S000002358 Hypothetical protein ORF Uncharacterized viable YDL200C S000002359 MGT1 DNA repair methyltransfer