Conservation of essential genes

Phenotype data for mouse (downloaded from MGI) and yeast (downloaded from SGD), were used to determined whether one, both, or neither genes are essential in ORTHOMCL families that have unambiguous (1:1:1) otholog families for yeast, mouse, and human.

Counts:

Total # of families with perfect 1:1:1 orthologs: 929
Both (yeast-mouse) essential: 24, only yeast: 4, only mouse: 47, neither: 32
No yeast data: 33, no mouse: 774, bad/deprecated mouse ID: 30

Data file:

essential_yeast_mouse_human.txt: contains all data below in a tab-delimited text file readable by Excel.

Mouse / yeast phenotypesOrthoMCL link (Family: Yeast ORF, Human ENSEMBL ID)Yeast ORF (SGD link)Yeast gene descriptionMouse gene (MGI link) Mouse gene description
Both essentialORTHOMCL1856: YAR007C, ENSP00000254719 YAR007CRFA1: Subunit of heterotrimeric Replication Factor A (RF-A), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombinationMGI:1915525Rpa1: replication protein A1  
Both essentialORTHOMCL3134: YBR136W, ENSP00000343741 YBR136WMEC1: Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; monitors and participates in meiotic recombinationMGI:108028Atr: ataxia telangiectasia and Rad3 related  
Both essentialORTHOMCL4160: YBR243C, ENSP00000346142 YBR243CALG7: UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycinMGI:1196396Dpagt1: dolichyl-phosphate (UDP-N-acetylglucosamine) acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase)  
Both essentialORTHOMCL4378: YDL132W, ENSP00000326804 YDL132WCDC53: Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradationMGI:1349658Cul1: cullin 1  
Both essentialORTHOMCL3310: YDR047W, ENSP00000246337 YDR047WHEM12: Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; activity inhibited by Cu2+, Zn2+, Fe2+, Fe3+ and sulfhydryl-specific reagentsMGI:98916Urod: uroporphyrinogen decarboxylase  
Both essentialORTHOMCL4169: YDR167W, ENSP00000299424 YDR167WTAF10: Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modificationMGI:1346320Taf10: TAF10 RNA polymerase II, TATA box binding protein (TBP)-associated factor  
Both essentialORTHOMCL5219: YDR394W, ENSP00000157812 YDR394WRPT3: One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase BMGI:1346093Psmc4: proteasome (prosome, macropain) 26S subunit, ATPase, 4  
Both essentialORTHOMCL4480: YDR460W, ENSP00000261245 YDR460WTFB3: Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunitMGI:106207Mnat1: menage a trois 1  
Both essentialORTHOMCL5602: YER112W, ENSP00000252816 YER112WLSM4: Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNAMGI:1354692Lsm4: LSM4 homolog, U6 small nuclear RNA associated (S. cerevisiae)  
Both essentialORTHOMCL3526: YER171W, ENSP00000221481 YER171WRAD3: 5' to 3' DNA helicase, involved in nucleotide excision repair and transcription; subunit of RNA polymerase II transcription initiation factor TFIIH; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD proteinMGI:95413Ercc2: excision repair cross-complementing rodent repair deficiency, complementation group 2  
Both essentialORTHOMCL2304: YGL001C, ENSP00000276346 YGL001CERG26: C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesisMGI:1099438Nsdhl: NAD(P) dependent steroid dehydrogenase-like  
Both essentialORTHOMCL3333: YGR098C, ENSP00000257934 YGR098CESP1: Separase with cysteine protease activity (related to caspases) that promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibited by Pds1pMGI:2146156Espl1: extra spindle poles-like 1 (S. cerevisiae)  
Both essentialORTHOMCL2799: YGR211W, ENSP00000227322 YGR211WZPR1: Essential protein with two zinc fingers, present in the nucleus of growing cells but relocates to the cytoplasm in starved cells via a process mediated by Cpr1p; binds to translation elongation factor eEF-1 (Tef1p)MGI:1330262Zfp259: zinc finger protein 259  
Both essentialORTHOMCL6931: YHR190W, ENSP00000220584 YHR190WERG9: Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathwayMGI:102706Fdft1: farnesyl diphosphate farnesyl transferase 1  
Both essentialORTHOMCL3661: YKL125W, ENSP00000198767 YKL125WRRN3: Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNAMGI:1925255Rrn3: RRN3 RNA polymerase I transcription factor homolog (yeast)  
Both essentialORTHOMCL6165: YLR103C, ENSP00000263201 YLR103CCDC45: DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromesMGI:1338073Cdc45l: cell division cycle 45 homolog (S. cerevisiae)-like  
Both essentialORTHOMCL5311: YLR347C, ENSP00000290158 YLR347CKAP95: Karyopherin beta, forms a dimeric complex with Srp1p (Kap60p) that mediates nuclear import of cargo proteins via a nuclear localization signal (NLS), interacts with nucleoporins to guide transport across the nuclear pore complexMGI:107532Kpnb1: karyopherin (importin) beta 1  
Both essentialORTHOMCL6998: YMR043W, ENSP00000265354 YMR043WMCM1: Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexesMGI:106658Srf: serum response factor  
Both essentialORTHOMCL3968: YOR103C, ENSP00000250498 YOR103COST2: Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteinsMGI:101912Dad1: defender against cell death 1  
Both essentialORTHOMCL3940: YOR117W, ENSP00000298852 YOR117WRPT5: One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcriptionMGI:1098754Psmc3: proteasome (prosome, macropain) 26S subunit, ATPase 3  
Both essentialORTHOMCL2677: YOR176W, ENSP00000262093 YOR176WHEM15: Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway; Yfh1p mediates the use of iron by Hem15pMGI:95513Fech: ferrochelatase  
Both essentialORTHOMCL5096: YOR261C, ENSP00000219313 YOR261CRPN8: Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11pMGI:1351511Psmd7: proteasome (prosome, macropain) 26S subunit, non-ATPase, 7  
Both essentialORTHOMCL6782: YOR278W, ENSP00000344508 YOR278WHEM4: Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in the heme biosynthetic pathwayMGI:98917Uros: uroporphyrinogen III synthase  
Both essentialORTHOMCL4273: YPL175W, ENSP00000297902 YPL175WSPT14: UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteinsMGI:99461Piga: phosphatidylinositol glycan anchor biosynthesis, class A  
Mouse only essentialORTHOMCL2822: YAL058W, ENSP00000247461YAL058WCNE1: Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeastMGI:88261Canx: calnexin  
Mouse only essentialORTHOMCL8072: YBL088C, ENSP00000278616YBL088CTEL1: Protein kinase, primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; homolog of human ataxia telangiectasia (ATM) geneMGI:107202Atm: ataxia telangiectasia mutated homolog (human)  
Mouse only essentialORTHOMCL16679: YBR023C, ENSP00000306991YBR023CCHS3: Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosanMGI:107821Has2: hyaluronan synthase 2  
Mouse only essentialORTHOMCL8947: YBR094W, ENSP00000233336YBR094W: Hypothetical proteinMGI:1916987Ttl: tubulin tyrosine ligase  
Mouse only essentialORTHOMCL3954: YCL008C, ENSP00000251968YCL008CSTP22: Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotypeMGI:106581Tsg101: tumor susceptibility gene 101  
Mouse only essentialORTHOMCL10198: YCR065W, ENSP00000333188YCR065WHCM1: Forkhead transcription factor that drives S-phase specific expression of genes involved in chromosome segregation, spindle dynamics, and budding; suppressor of calmodulin mutants with specific SPB assembly defects; telomere maintenance roleMGI:1349428Foxl2: forkhead box L2  
Mouse only essentialORTHOMCL5362: YDL090C, ENSP00000246166YDL090CRAM1: Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunitMGI:1861305Fntb: farnesyltransferase, CAAX box, beta  
Mouse only essentialORTHOMCL4807: YDR027C, ENSP00000272322YDR027CVPS54: Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; potentially phosphorylated by Cdc28pMGI:2178798Vps54: vacuolar protein sorting 54 (yeast)  
Mouse only essentialORTHOMCL7529: YDR142C, ENSP00000315680YDR142CPEX7: Peroxisomal signal receptor for the N-terminal nonapeptide signal (PTS2) of peroxisomal matrix proteins; WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP)MGI:1321392Pex7: peroxisome biogenesis factor 7  
Mouse only essentialORTHOMCL3584: YDR410C, ENSP00000313340YDR410CSTE14: Farnesyl cysteine-carboxyl methyltransferase, mediates the carboxyl methylation step during C-terminal CAAX motif processing of a-factor and RAS proteins in the endoplasmic reticulum, localizes to the ER membrane MGI:1888594Icmt: isoprenylcysteine carboxyl methyltransferase  
Mouse only essentialORTHOMCL6977: YDR465C, ENSP00000252288YDR465CRMT2: Arginine methyltransferase; ribosomal protein L12 is a substrateMGI:1098221Gamt: guanidinoacetate methyltransferase  
Mouse only essentialORTHOMCL2959: YDR486C, ENSP00000223500YDR486CVPS60: Cytoplasmic and vacuolar membrane protein involved in late endosome to vacuole transport; required for normal filament maturation during pseudohyphal growth; may function in targeting specific cargo proteins for degradationMGI:1924209Chmp5: chromatin modifying protein 5  
Mouse only essentialORTHOMCL12009: YEL009C, ENSP00000252818YEL009CGCN4: Transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levelsMGI:96648Jund1: Jun proto-oncogene related gene d1  
Mouse only essentialORTHOMCL3353: YER095W, ENSP00000267868YER095WRAD51: Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA proteinMGI:97890Rad51: RAD51 homolog (S. cerevisiae)  
Mouse only essentialORTHOMCL6240: YER107C, ENSP00000217117YER107CGLE2: Component of the nuclear pore complex required for polyadenylated RNA export but not for protein import, homologous to S. pombe Rae1pMGI:1913929Rae1: RAE1 RNA export 1 homolog (S. pombe)  
Mouse only essentialORTHOMCL2761: YFL018C, ENSP00000205402YFL018CLPD1: Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexesMGI:107450Dld: dihydrolipoamide dehydrogenase  
Mouse only essentialORTHOMCL10610: YFR038W, ENSP00000239027YFR038WIRC5: Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52 fociMGI:106209Hells: helicase, lymphoid specific  
Mouse only essentialORTHOMCL6662: YGL043W, ENSP00000353558YGL043WDST1: General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sitesMGI:1196624Tcea1: transcription elongation factor A (SII) 1  
Mouse only essentialORTHOMCL6801: YGL153W, ENSP00000270774YGL153WPEX14: Peroxisomal membrane protein that is a central component of the peroxisomal protein import machinery, interacts with PTS1 (Pex5p) and PTS2 (Pex7p) peroxisomal matrix protein signal recognition factors and membrane receptor Pex13pMGI:1927868Pex14: peroxisomal biogenesis factor 14  
Mouse only essentialORTHOMCL5579: YGR112W, ENSP00000277404YGR112WSHY1: Mitochondrial inner membrane protein required for normal respiration, possible chaperone involved in assembly of cytochrome c oxidase; similar to SURF1 from mammals, chickens, and D. melanogasterMGI:98443Surf1: surfeit gene 1  
Mouse only essentialORTHOMCL1995: YGR132C, ENSP00000300408YGR132CPHB1: Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregationMGI:97572Phb: prohibitin  
Mouse only essentialORTHOMCL3265: YIL049W, ENSP00000264228YIL049WDFG10: Protein of unknown function, involved in filamentous growthMGI:1930252Srd5a2l: steroid 5 alpha-reductase 2-like  
Mouse only essentialORTHOMCL8419: YJL088W, ENSP00000039007YJL088WARG3: Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithineMGI:97448Otc: ornithine transcarbamylase  
Mouse only essentialORTHOMCL5253: YJL208C, ENSP00000300491YJL208CNUC1: Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has a role in mitochondrial recombinationMGI:1261433Endog: endonuclease G  
Mouse only essentialORTHOMCL5644: YKL113C, ENSP00000305480YKL113CRAD27: 5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 familyMGI:102779Fen1: flap structure specific endonuclease 1  
Mouse only essentialORTHOMCL4990: YKR009C, ENSP00000256216YKR009CFOX2: Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activitiesMGI:105089Hsd17b4: hydroxysteroid (17-beta) dehydrogenase 4  
Mouse only essentialORTHOMCL4051: YLL009C, ENSP00000261070YLL009CCOX17: Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidaseMGI:1333806Cox17: cytochrome c oxidase, subunit XVII assembly protein homolog (yeast)  
Mouse only essentialORTHOMCL3830: YLR447C, ENSP00000290949YLR447CVMA6: Subunit D of the five-subunit V0 integral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found in the endomembrane system; stabilizes VO subunits; required for V1 domain assembly on the vacuolar membraneMGI:1201778Atp6v0d1: ATPase, H+ transporting, lysosomal V0 subunit D1  
Mouse only essentialORTHOMCL1153: YML012W, ENSP00000303145YML012WERV25: Protein that forms a heterotrimeric complex with Erp1, Erp2p, and Emp24, member of the p24 family involved in endoplasmic reticulum to Golgi transportMGI:1915831Tmed10: transmembrane emp24-like trafficking protein 10 (yeast)  
Mouse only essentialORTHOMCL4311: YML097C, ENSP00000284957YML097CVPS9: A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitinMGI:1929459Rabgef1: RAB guanine nucleotide exchange factor (GEF) 1  
Mouse only essentialORTHOMCL5327: YMR080C, ENSP00000262803YMR080CNAM7: ATP-dependent RNA helicase of the SFI superfamily, required for nonsense mediated mRNA decay and for efficient translation termination at nonsense codons; involved in telomere maintenanceMGI:107995Upf1: UPF1 regulator of nonsense transcripts homolog (yeast)  
Mouse only essentialORTHOMCL6519: YMR154C, ENSP00000253693YMR154CRIM13: Calpain-like protease involved in proteolytic activation of Rim101p in response to alkaline pH; has similarity to A. nidulans palBMGI:1338030Capn7: calpain 7  
Mouse only essentialORTHOMCL7426: YMR201C, ENSP00000259463YMR201CRAD14: Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA proteinMGI:99135Xpa: xeroderma pigmentosum, complementation group A  
Mouse only essentialORTHOMCL4702: YMR234W, ENSP00000313350YMR234WRNH1: Ribonuclease H1, removes RNA primers during Okazaki fragment synthesis; degrades RNA attached to the 5'-end of a DNA strandMGI:1335073Rnaseh1: ribonuclease H1  
Mouse only essentialORTHOMCL5136: YMR274C, ENSP00000309163YMR274CRCE1: Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromoneMGI:1336895Rce1: RCE1 homolog, prenyl protein peptidase (S. cerevisiae)  
Mouse only essentialORTHOMCL6271: YNL141W, ENSP00000352331YNL141WAAH1: Adenine deaminase (adenine aminohydrolase), involved in purine salvage and nitrogen catabolismMGI:87916Ada: adenosine deaminase  
Mouse only essentialORTHOMCL7462: YNL239W, ENSP00000261714YNL239WLAP3: Aminopeptidase of cysteine protease family, has a DNA binding activity and acts as bleomycin hydrolase in vitro; transcription is regulated by galactose via Gal4pMGI:1345186Blmh: bleomycin hydrolase  
Mouse only essentialORTHOMCL1386: YNL259C, ENSP00000316854YNL259CATX1: Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptakeMGI:1333855Atox1: ATX1 (antioxidant protein 1) homolog 1 (yeast)  
Mouse only essentialORTHOMCL5733: YOL031C, ENSP00000265195YOL031CSIL1: Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like proteinMGI:1932040Sil1: endoplasmic reticulum chaperone SIL1 homolog (S. cerevisiae)  
Mouse only essentialORTHOMCL9550: YOL147C, ENSP00000300056YOL147CPEX11: Peroxisomal membrane protein required for peroxisome proliferation and medium-chain fatty acid oxidation, most abundant protein in the peroxisomal membrane, regulated by Adr1p and Pip2p-Oaf1p, promoter contains ORE and UAS1-like elementsMGI:1338788Pex11a: peroxisomal biogenesis factor 11a  
Mouse only essentialORTHOMCL3621: YOR196C, ENSP00000261434YOR196CLIP5: Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthaseMGI:1934604Lias: lipoic acid synthetase  
Mouse only essentialORTHOMCL2179: YOR269W, ENSP00000006951YOR269WPAC1: Protein involved in nuclear migration, part of the dynein/dynactin pathway; targets dynein to microtubule tips, which is necessary for sliding of microtubules along bud cortex; synthetic lethal with bni1; homolog of human LIS1MGI:109520Pafah1b1: platelet-activating factor acetylhydrolase, isoform 1b, beta1 subunit  
Mouse only essentialORTHOMCL4274: YPL022W, ENSP00000310520YPL022WRAD1: Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF proteinMGI:1354163Ercc4: excision repair cross-complementing rodent repair deficiency, complementation group 4  
Mouse only essentialORTHOMCL3733: YPL138C, ENSP00000285106YPL138CSPP1: Subunit of COMPASS (Set1C), a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding proteinMGI:1921572Cxxc1: CXXC finger 1 (PHD domain)  
Mouse only essentialORTHOMCL5197: YPL149W, ENSP00000263322YPL149WATG5: Conserved autophagy-related protein that undergoes conjugation with Atg12p and then associates with Atg16p to form a cytosolic complex essential for autophagosome formationMGI:1277186Atg5: autophagy-related 5 (yeast)  
Mouse only essentialORTHOMCL4699: YPL227C, ENSP00000239891YPL227CALG5: UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulumMGI:1913498Alg5: asparagine-linked glycosylation 5 homolog (yeast, dolichyl-phosphate beta-glucosyltransferase)  
Mouse only essentialORTHOMCL3076: YPL259C, ENSP00000291439YPL259CAPM1: Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sortingMGI:102776Ap1m1: adaptor-related protein complex AP-1, mu subunit 1  
Neither essentialORTHOMCL6156: YBL019W, ENSP00000306132YBL019WAPN2: Class II abasic (AP) endonuclease involved in repair of DNA damage; homolog of human HAP1 and E. coli exoIIIMGI:1924872Apex2: apurinic/apyrimidinic endonuclease 2  
Neither essentialORTHOMCL2473: YBL064C, ENSP00000236251YBL064CPRX1: Mitochondrial peroxiredoxin (1-Cys Prx) with thioredoxin peroxidase activity, has a role in reduction of hydroperoxides; induced during respiratory growth and under conditions of oxidative stressMGI:894320Prdx6: peroxiredoxin 6  
Neither essentialORTHOMCL10670: YBR276C, ENSP00000239223YBR276CPPS1: Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycleMGI:105120Dusp1: dual specificity phosphatase 1  
Neither essentialORTHOMCL3323: YCR075C, ENSP00000046640YCR075CERS1: Protein with similarity to human cystinosin, which is a H(+)-driven transporter involved in L-cystine export from lysosomes and implicated in the disease cystinosis; contains seven transmembrane domainsMGI:1932872Ctns: cystinosis, nephropathic  
Neither essentialORTHOMCL6885: YCR088W, ENSP00000258780YCR088WABP1: Actin-binding protein of the cortical actin cytoskeleton, important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organizationMGI:700006Dbnl: drebrin-like  
Neither essentialORTHOMCL4391: YDL168W, ENSP00000296412YDL168WSFA1: Bifunctional enzyme containing both alcohol dehydrogenase and glutathione-dependent formaldehyde dehydrogenase activities, functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1pMGI:87929Adh5: alcohol dehydrogenase 5 (class III), chi polypeptide  
Neither essentialORTHOMCL5194: YDR097C, ENSP00000234420YDR097CMSH6: Protein required for mismatch repair in mitosis and meiosis, forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; potentially phosphorylated by Cdc28pMGI:1343961Msh6: mutS homolog 6 (E. coli)  
Neither essentialORTHOMCL6416: YDR159W, ENSP00000291688YDR159WSAC3: Nuclear pore-associated protein, forms a complex with Thp1p that is involved in transcription and in mRNA export from the nucleusMGI:1930089Mcm3ap: minichromosome maintenance deficient 3 (S. cerevisiae) associated protein  
Neither essentialORTHOMCL1098: YDR256C, ENSP00000241052YDR256CCTA1: Catalase A, breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidationMGI:88271Cat: catalase  
Neither essentialORTHOMCL6374: YDR283C, ENSP00000263791YDR283CGCN2: Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complexMGI:1353427Eif2ak4: eukaryotic translation initiation factor 2 alpha kinase 4  
Neither essentialORTHOMCL6522: YDR305C, ENSP00000342087YDR305CHNT2: Dinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the histidine triad (HIT) superfamily of nucleotide-binding proteinsMGI:1277947Fhit: fragile histidine triad gene  
Neither essentialORTHOMCL3127: YDR386W, ENSP00000307853YDR386WMUS81: Helix-hairpin-helix protein, involved in DNA repair and replication fork stability; functions as an endonuclease in complex with Mms4p; interacts with Rad54pMGI:1918961Mus81: MUS81 endonuclease homolog (yeast)  
Neither essentialORTHOMCL6492: YDR419W, ENSP00000306591YDR419WRAD30: DNA polymerase eta, involved in the predominantly error-free bypass replication of DNA lesions, catalyzes the efficient and accurate synthesis of DNA opposite cyclobutane pyrimidine dimers; homolog of human POLH and bacterial DinB proteinsMGI:1891457Polh: polymerase (DNA directed), eta (RAD 30 related)  
Neither essentialORTHOMCL3744: YER007W, ENSP00000264180YER007WPAC2: Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomylMGI:1917680Tbce: tubulin-specific chaperone e  
Neither essentialORTHOMCL7626: YER019W, ENSP00000258052YER019WISC1: Inositol phosphosphingolipid phospholipase C, hydrolyzes inositolphosphosphingolipids, activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol, mediates Na+ and Li+ halotolerance, contains a P loop-like domainMGI:1278330Smpd2: sphingomyelin phosphodiesterase 2, neutral  
Neither essentialORTHOMCL1554: YER042W, ENSP00000313921YER042WMXR1: Peptide methionine sulfoxide reductase, reverses the oxidation of methionine residues; involved in oxidative damage repair, providing resistance to oxidative stress and regulation of lifespanMGI:106916Msra: methionine sulfoxide reductase A  
Neither essentialORTHOMCL8565: YER179W, ENSP00000216024YER179WDMC1: Meiosis-specific protein required for repair of double-strand breaks and pairing between homologous chromosomes; homolog of Rad51p and the bacterial RecA proteinMGI:105393Dmc1h: disrupted meiotic cDNA 1 homolog  
Neither essentialORTHOMCL4130: YFR010W, ENSP00000261601YFR010WUBP6: Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; deletion causes hypersensitivity to cycloheximide and other toxic compoundsMGI:1928898Usp14: ubiquitin specific peptidase 14  
Neither essentialORTHOMCL10131: YGL033W, ENSP00000253789YGL033WHOP2: Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repairMGI:1098610Psmc3ip: proteasome (prosome, macropain) 26S subunit, ATPase 3, interacting protein  
Neither essentialORTHOMCL4567: YGR123C, ENSP00000012443YGR123CPPT1: Protein serine/threonine phosphatase with similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growthMGI:102666Ppp5c: protein phosphatase 5, catalytic subunit  
Neither essentialORTHOMCL5487: YJL073W, ENSP00000218763YJL073WJEM1: DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2MGI:107373Dnajc3: DnaJ (Hsp40) homolog, subfamily C, member 3  
Neither essentialORTHOMCL6339: YJR117W, ENSP00000196805YJR117WSTE24: Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spansMGI:1890508Zmpste24: zinc metallopeptidase, STE24 homolog (S. cerevisiae)  
Neither essentialORTHOMCL4519: YKL017C, ENSP00000255078YKL017CHCS1: Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activitiesMGI:99954Ighmbp2: immunoglobulin mu binding protein 2  
Neither essentialORTHOMCL5642: YLR216C, ENSP00000303754YLR216CCPR6: Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activityMGI:1914988Ppid: peptidylprolyl isomerase D (cyclophilin D)  
Neither essentialORTHOMCL3547: YLR370C, ENSP00000228825YLR370CARC18: Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patchesMGI:1928375Arpc3: actin related protein 2/3 complex, subunit 3  
Neither essentialORTHOMCL1439: YLR389C, ENSP00000265986YLR389CSTE23: Metalloprotease involved, with homolog Axl1p, in N-terminal processing of pro-a-factor to the mature form; member of the insulin-degrading enzyme familyMGI:96412Ide: insulin degrading enzyme  
Neither essentialORTHOMCL3732: YLR396C, ENSP00000267199YLR396CVPS33: ATP-binding protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at both the Golgi-to-endosome and endosome-to-vacuole stages of protein transportMGI:1924823Vps33a: vacuolar protein sorting 33A (yeast)  
Neither essentialORTHOMCL4618: YMR167W, ENSP00000231790YMR167WMLH1: Protein required for mismatch repair in mitosis and meiosis, postmeiotic segregation, and spore viability; forms a complex with Pms1p and Msh2p to repair mismatched DNA; human homolog is associated with hereditary non-polyposis colon cancerMGI:101938Mlh1: mutL homolog 1 (E. coli)  
Neither essentialORTHOMCL2040: YNL045W, ENSP00000228740YNL045W: Leucyl aminopeptidase (leukotriene A4 hydrolase) with epoxide hydrolase activity, metalloenzyme containing one zinc atom; role in vivo is not defined; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleusMGI:96836Lta4h: leukotriene A4 hydrolase  
Neither essentialORTHOMCL2628: YOL075C, ENSP00000260645YOL075C: Hypothetical proteinMGI:1351659Abcg5: ATP-binding cassette, sub-family G (WHITE), member 5  
Neither essentialORTHOMCL2624: YOL090W, ENSP00000233146YOL090WMSH2: Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity; Msh2p-Msh6p binds to and hydrolyzes ATPMGI:101816Msh2: mutS homolog 2 (E. coli)  
Neither essentialORTHOMCL5220: YPL172C, ENSP00000261643YPL172CCOX10: Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disordersMGI:1917633Cox10: COX10 homolog, cytochrome c oxidase assembly protein, heme A: farnesyltransferase (yeast)  
No systematic deletion data for mouse, yeast is essentialORTHOMCL3823: YAL025C, ENSP00000353246YAL025CMAK16: Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for normal concentration of free 60S ribosomal subunits; required for maintenance of M1 satellite double-stranded RNA of the L-A virusMGI:1915170  
No systematic deletion data for mouse, yeast is essentialORTHOMCL4848: YAL043C, ENSP00000343851YAL043CPTA1: Subunit of holo-CPF, a multiprotein complex and functional homolog of mammalian CPSF, required for the cleavage and polyadenylation of mRNA and snoRNA 3' ends; involved in pre-tRNA processing; binds to the phosphorylated CTD of RNAPIIMGI:1915438  
No systematic deletion data for mouse, yeast is essentialORTHOMCL10525: YAR008W, ENSP00000305524YAR008WSEN34: Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonucleaseMGI:1913328  
No systematic deletion data for mouse, yeast is essentialORTHOMCL4949: YBL004W, ENSP00000261637YBL004WUTP20: Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNAMGI:1917933  
No systematic deletion data for mouse, yeast is essentialORTHOMCL3909: YBL023C, ENSP00000265056YBL023CMCM2: Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complexMGI:105380  
No systematic deletion data for mouse, yeast is essentialORTHOMCL4367: YBL035C, ENSP00000265465YBL035CPOL12: B subunit of DNA polymerase alpha-primase complex, required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulationMGI:99690  
No systematic deletion data for mouse, yeast is essentialORTHOMCL3880: YBL041W, ENSP00000262193YBL041WPRE7: 20S proteasome beta-type subunitMGI:104884  
No systematic deletion data for mouse, yeast is essentialORTHOMCL3523: YBL074C, ENSP00000313674YBL074CAAR2: Component of the U5 snRNP, required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistronMGI:1915545  
No systematic deletion data for mouse, yeast is essentialORTHOMCL3105: YBL076C, ENSP00000354047YBL076CILS1: Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin AMGI:2145219  
No systematic deletion data for mouse, yeast is essentialORTHOMCL2818: YBL084C, ENSP00000066544YBL084CCDC27: Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transitionMGI:102685  
No systematic deletion data for mouse, yeast is essentialORTHOMCL1942: YBL092W, ENSP00000339064YBL092WRPL32: Protein component of the large (60S) ribosomal subunit, has similarity to rat L32 ribosomal protein; overexpression disrupts telomeric silencingMGI:98038  
No systematic deletion data for mouse, yeast is essentialORTHOMCL5090: YBL097W, ENSP00000240423YBL097WBRN1: Essential protein required for chromosome condensation, likely to function as an intrinsic component of the condensation machinery, may influence multiple aspects of chromosome transmission and dynamicsMGI:2444777  
No systematic deletion data for mouse, yeast is essentialORTHOMCL3280: YBR004C, ENSP00000078527YBR004CGPI18: Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondriaMGI:2442480  
No systematic deletion data for mouse, yeast is essentialORTHOMCL5047: YBR055C, ENSP00000266079YBR055CPRP6: Splicing factor, component of the U4/U6-U5 snRNP complexMGI:1922946  
No systematic deletion data for mouse, yeast is essentialORTHOMCL5563: YBR060C, ENSP00000234296YBR060CORC2: Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; may be phosphorylated by Cdc28pMGI:1328306  
No systematic deletion data for mouse, yeast is essentialORTHOMCL5390: YBR070C, ENSP00000303404YBR070CALG14: Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferasesMGI:1914039  
No systematic deletion data for mouse, yeast is essentialORTHOMCL4217: YBR079C, ENSP00000263141YBR079CRPG1: Subunit of the core complex of translation initiation factor 3(eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomesMGI:95301  
No systematic deletion data for mouse, yeast is essentialORTHOMCL4537: YBR080C, ENSP00000225282YBR080CSEC18: ATPase required for the release of Sec17p during the 'priming' step in homotypic vacuole fusion and for ER to Golgi transport; homolog of the mammalian NSFMGI:104560  
No systematic deletion data for mouse, yeast is essentialORTHOMCL4485: YBR087W, ENSP00000255484YBR087WRFC5: Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilonMGI:1916513  
No systematic deletion data for mouse, yeast is essentialORTHOMCL2821: YBR088C, ENSP00000254983YBR088CPOL30: Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repairMGI:97503  
No systematic deletion data for mouse, yeast is essentialORTHOMCL5154: YBR102C, ENSP00000353564YBR102CEXO84: Essential protein with dual roles in spliceosome assembly and exocytosis; the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p) mediates polarized targeting of secretory vesicles to active sites of exocytosisMGI:2142527  
No systematic deletion data for mouse, yeast is essentialORTHOMCL5442: YBR110W, ENSP00000262374YBR110WALG1: Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human orthologMGI:2384774  
No systematic deletion data for mouse, yeast is essentialORTHOMCL5036: YBR123C, ENSP00000277434YBR123CTFC1: One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63MGI:1917489  
No systematic deletion data for mouse, yeast is essentialORTHOMCL5488: YBR142W, ENSP00000328690YBR142WMAK5: Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunitsMGI:1351337  
No systematic deletion data for mouse, yeast is essentialORTHOMCL5074: YBR154C, ENSP00000215587YBR154CRPB5: RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivationMGI:1913670  
No systematic deletion data for mouse, yeast is essentialORTHOMCL5953: YBR192W, ENSP00000320688YBR192WRIM2: Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier familyMGI:1924909  
No systematic deletion data for mouse, yeast is essentialORTHOMCL4679: YBR247C, ENSP00000230340YBR247CENP1: Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolusMGI:1858419  
No systematic deletion data for mouse, yeast is essentialORTHOMCL2753: YBR257W, ENSP00000221770YBR257WPOP4: Subunit of both RNase MRP, which cleaves pre-rRNA, and nuclear RNase P, which cleaves tRNA precursors to generate mature 5' ends; binds to the RPR1 RNA subunit in Rnase PMGI:1913411  
No systematic deletion data for mouse, yeast is essentialORTHOMCL6448: YCL004W, ENSP00000262764YCL004WPGS1: Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesisMGI:1921701  
No systematic deletion data for mouse, yeast is essentialORTHOMCL5186: YCL017C, ENSP00000262876YCL017CNFS1: Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis; required for the post-transcriptional thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondriaMGI:1316706  
No systematic deletion data for mouse, yeast is essentialORTHOMCL5505: YCL059C, ENSP00000229214YCL059CKRR1: Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunitMGI:1289274  
No systematic deletion data for mouse, yeast is essentialORTHOMCL4585: YCR054C, ENSP00000252934YCR054CCTR86: Essential protein of unknown functionMGI:1859293  
No systematic deletion data for mouse, yeast is essentialORTHOMCL4443: YCR057C, ENSP00000291576YCR057CPWP2: Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesisMGI:1341200  
No systematic deletion data for mouse, yeast is essentialORTHOMCL2732: YCR093W, ENSP00000320949YCR093WCDC39: Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factorMGI:2442402  
No systematic deletion data for mouse, yeast is essentialORTHOMCL5528: YDL004W, ENSP00000215375YDL004WATP16: Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesisMGI:1913293  
No systematic deletion data for mouse, yeast is essentialORTHOMCL4003: YDL007W, ENSP00000261303YDL007WRPT2: One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particleMGI:106054  
No systematic deletion data for mouse, yeast is essentialORTHOMCL3445: YDL015C, ENSP00000215567YDL015CTSC13: Enoyl reductase that catalyzes the last step in each cycle of very long chain fatty acid elongation, localizes to the ER, highly enriched in a structure marking nuclear-vacuolar junctions, coimmunoprecipitates with elongases Fen1p and Sur4pMGI:1915408  
No systematic deletion data for mouse, yeast is essentialORTHOMCL8268: YDL028C, ENSP00000230510YDL028CMPS1: Dual-specificity kinase required for spindle pole body (SPB) duplication and spindle checkpoint function; substrates include SPB proteins Spc42p, Spc110p, and Spc98p, mitotic exit network protein Mob1p, and checkpoint protein Mad1pMGI:1194921  
No systematic deletion data for mouse, yeast is essentialORTHOMCL6810: YDL029W, ENSP00000260641YDL029WARP2: Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarityMGI:1913963  
No systematic deletion data for mouse, yeast is essentialORTHOMCL6146: YDL030W, ENSP00000329527YDL030WPRP9: Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complexMGI:1922312  
No systematic deletion data for mouse, yeast is essentialORTHOMCL4429: YDL031W, ENSP00000304072YDL031WDBP10: Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesisMGI:1919240  
No systematic deletion data for mouse, yeast is essentialORTHOMCL4433: YDL060W, ENSP00000301364YDL060WTSR1: Protein required for processing of 20S pre-rRNA in the cytoplasm, associates with pre-40S ribosomal particlesMGI:2144566  
No systematic deletion data for mouse, yeast is essentialORTHOMCL3199: YDL097C, ENSP00000261712YDL097CRPN6: Essential, non-ATPase regulatory subunit of the 26S proteasome lid required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletionMGI:1916327  
No systematic deletion data for mouse, yeast is essentialORTHOMCL5538: YDL098C, ENSP00000274712YDL098CSNU23: Component of U4/U6.U5 snRNP involved in mRNA splicing via spliceosomeMGI:1913742  
No systematic deletion data for mouse, yeast is essentialORTHOMCL6972: YDL105W, ENSP00000263449YDL105WNSE4: Nuclear protein that plays a role in the function of the Smc5p-Rhc18p complexMGI:1915122  
No systematic deletion data for mouse, yeast is essentialORTHOMCL3995: YDL111C, ENSP00000265564YDL111CRRP42: Protein involved in rRNA processing; component of the exosome 3->5 exonuclease complex with Rrp4p, Rrp41p, Rrp43p and Dis3pMGI:1913696  
No systematic deletion data for mouse, yeast is essentialORTHOMCL5223: YDL143W, ENSP00000233836YDL143WCCT4: Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivoMGI:104689  
No systematic deletion data for mouse, yeast is essentialORTHOMCL2570: YDL147W, ENSP00000348442YDL147WRPN5: Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7pMGI:1914247  
No systematic deletion data for mouse, yeast is essentialORTHOMCL6751: YDL148C, ENSP00000315674YDL148CNOP14: Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNAMGI:1922666  
No systematic deletion data for mouse, yeast is essentialORTHOMCL5519: YDL205C, ENSP00000278715YDL205CHEM3: Phorphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3pMGI:96112  
No systematic deletion data for mouse, yeast is essentialORTHOMCL4730: YDL208W, ENSP00000274606YDL208WNHP2: Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processingMGI:1098547  
No systematic deletion data for mouse, yeast is essentialORTHOMCL4836: YDL217C, ENSP00000320236YDL217CTIM22: Component of the mitochondrial Tim54p-Tim22p complex involved in insertion of polytopic proteins into the inner membraneMGI:1929742  
No systematic deletion data for mouse, yeast is essentialORTHOMCL6636: YDR013W, ENSP00000262460YDR013WPSF1: Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machineryMGI:1916520  
No systematic deletion data for mouse, yeast is essentialORTHOMCL3804: YDR021W, ENSP00000269349YDR021WFAL1: Nucleolar protein required for maturation of 18S rRNA, member of the eIF4A subfamily of DEAD-box ATP-dependent RNA helicasesMGI:1923731  
No systematic deletion data for mouse, yeast is essentialORTHOMCL3551: YDR044W, ENSP00000264193YDR044WHEM13: Coproporphyrinogen III oxidase, an oxygen requiring enzyme that catalyzes the sixth step in the heme biosynthetic pathway; localizes to the mitochondrial inner membrane; transcription is repressed by oxygen and heme (via Rox1p and Hap1p)MGI:104841  
No systematic deletion data for mouse, yeast is essentialORTHOMCL4578: YDR045C, ENSP00000293860YDR045CRPC11: RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcriptionMGI:1914255  
No systematic deletion data for mouse, yeast is essentialORTHOMCL5246: YDR054C, ENSP00000263228YDR054CCDC34: Ubiquitin-conjugating enzyme or E2; together with Skp1p, Rbx1p, Cdc53p, and an F-box protein, forms a ubiquitin-protein ligase called the SCF complex which regulates cell cycle progression by targeting key substrates for degradationMGI:1914865  
No systematic deletion data for mouse, yeast is essentialORTHOMCL5731: YDR060W, ENSP00000234170YDR060WMAK21: Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding proteinMGI:109386  
No systematic deletion data for mouse, yeast is essentialORTHOMCL3041: YDR062W, ENSP00000216484YDR062WLCB2: Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganineMGI:108074  
No systematic deletion data for mouse, yeast is essentialORTHOMCL13066: YDR081C, ENSP00000355162YDR081CPDC2: Transcription factor required for the synthesis of the glycolytic enzyme pyruvate decarboxylase, required for high level expression of both the THI and the PDC genesMGI:2685264  
No systematic deletion data for mouse, yeast is essentialORTHOMCL2921: YDR088C, ENSP00000297151YDR088CSLU7: RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domainMGI:2385598  
No systematic deletion data for mouse, yeast is essentialORTHOMCL3687: YDR091C, ENSP00000296577YDR091CRLI1: Essential iron-sulfur protein required for ribosome biogenesis and translation initiation; facilitates binding of a multifactor complex (MFC) of translation initiation factors to the small ribosomal subunit; predicted ABC family ATPaseMGI:1195458  
No systematic deletion data for mouse, yeast is essentialORTHOMCL13063: YDR164C, ENSP00000221283YDR164CSEC1: Sm-like protein involved in docking and fusion of exocytic vesicles through binding to assembled SNARE complexes at the membrane; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE functionMGI:107370  
No systematic deletion data for mouse, yeast is essentialORTHOMCL8088: YDR168W, ENSP00000222005YDR168WCDC37: Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycleMGI:109531  
No systematic deletion data for mouse, yeast is essentialORTHOMCL4248: YDR177W, ENSP00000261427YDR177WUBC1: Ubiquitin-conjugating enzyme that mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress responseMGI:1858216  
No systematic deletion data for mouse, yeast is essentialORTHOMCL5069: YDR190C, ENSP00000318297YDR190CRVB1: Essential protein involved in transcription regulation; component of chromatin remodeling complexes; required for assembly and function of the INO80 complex; member of the RUVB-like protein familyMGI:1928760  
No systematic deletion data for mouse, yeast is essentialORTHOMCL4465: YDR196C, ENSP00000341504YDR196C: predicted to catalyze the final step in synthesis of Coenzyme AMGI:1915337  
No systematic deletion data for mouse, yeast is essentialORTHOMCL4142: YDR212W, ENSP00000239585YDR212WTCP1: Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in maintenance of actin cytoskeleton; homolog to Drosophila melanogaster and mouse tailless complex polypeptideMGI:98535  
No systematic deletion data for mouse, yeast is essentialORTHOMCL2286: YDR228C, ENSP00000298281YDR228CPCF11: mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p)MGI:1919579  
No systematic deletion data for mouse, yeast is essentialORTHOMCL5414: YDR236C, ENSP00000257452YDR236CFMN1: Riboflavin kinase, phosphorylates riboflavin to form riboflavin monophosphate (FMN), which is a necessary cofactor for many enzymes; localizes to microsomes and to the mitochondrial inner membraneMGI:1914688  
No systematic deletion data for mouse, yeast is essentialORTHOMCL3599: YDR238C, ENSP00000249923YDR238CSEC26: Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)MGI:1917599  
No systematic deletion data for mouse, yeast is essentialORTHOMCL5515: YDR246W, ENSP00000351896YDR246WTRS23: One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic; human homolog is TRAPPC4MGI:1926211  
No systematic deletion data for mouse, yeast is essentialORTHOMCL3077: YDR267C, ENSP00000272402YDR267CCIA1: Essential protein involved in assembly of cytosolic and nuclear iron-sulfur proteinsMGI:1346998  
No systematic deletion data for mouse, yeast is essentialORTHOMCL2926: YDR280W, ENSP00000243498YDR280WRRP45: Protein involved in rRNA processing; component of the exosome 3->5 exonuclease complexMGI:1355319  
No systematic deletion data for mouse, yeast is essentialORTHOMCL7867: YDR302W, ENSP00000281382YDR302WGPI11: ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homologMGI:99462  
No systematic deletion data for mouse, yeast is essentialORTHOMCL3542: YDR311W, ENSP00000265963YDR311WTFB1: Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activatorsMGI:1277216  
No systematic deletion data for mouse, yeast is essentialORTHOMCL3447: YDR325W, ENSP00000251496YDR325WYCG1: Non-SMC subunit of the condensin complex (Smc2p-Smc4p-Ycs4p-Brn1p-Ycg1p); required for establishment and maintenance of chromosome condensation, chromosome segregation and for chromatin binding of the condensin complexMGI:1930197  
No systematic deletion data for mouse, yeast is essentialORTHOMCL314: YDR328C, ENSP00000231487YDR328CSKP1: Evolutionarily conserved kinetochore protein that is part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPaseMGI:103575  
No systematic deletion data for mouse, yeast is essentialORTHOMCL4427: YDR365C, ENSP00000202816YDR365CESF1: Nucleolar protein involved in pre-rRNA processing; depletion causes severely decreased 18S rRNA levelsMGI:1913830  
No systematic deletion data for mouse, yeast is essentialORTHOMCL4733: YDR376W, ENSP00000293195YDR376WARH1: Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viabilityMGI:104724  
No systematic deletion data for mouse, yeast is essentialORTHOMCL4560: YDR390C, ENSP00000246548YDR390CUBA2: Nuclear protein that acts as a heterodimer with Aos1p to activate Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viabilityMGI:1858313  
No systematic deletion data for mouse, yeast is essentialORTHOMCL3627: YDR397C, ENSP00000236164YDR397CNCB2: Beta subunit of the NC2 dimeric histone-fold complex; represses RNA polymerase II transcription through binding to TBP and inhibition of TFIIA and TFIIB; homologous to the Dr1 subunit of the mammalian NC2 (negative cofactor2)MGI:1100515  
No systematic deletion data for mouse, yeast is essentialORTHOMCL5869: YDR398W, ENSP00000296126YDR398WUTP5: Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNAMGI:1919765  
No systematic deletion data for mouse, yeast is essentialORTHOMCL4257: YDR404C, ENSP00000301788YDR404CRPB7: RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4pMGI:1914960  
No systematic deletion data for mouse, yeast is essentialORTHOMCL2585: YDR427W, ENSP00000333811YDR427WRPN9: Non-ATPase regulatory subunit of the 26S proteasome, has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defectsMGI:1345192  
No systematic deletion data for mouse, yeast is essentialORTHOMCL5360: YDR434W, ENSP00000309430YDR434WGPI17: Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Sp homologMGI:2687325  
No systematic deletion data for mouse, yeast is essentialORTHOMCL6236: YDR449C, ENSP00000261708YDR449CUTP6: Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNAMGI:2445193  
No systematic deletion data for mouse, yeast is essentialORTHOMCL3681: YDR454C, ENSP00000317659YDR454CGUK1: Guanylate kinase, converts GMP to GDP; required for growth and mannose outer chain elongation of cell wall N-linked glycoproteinsMGI:95871  
No systematic deletion data for mouse, yeast is essential